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2015 Vol.33, Issue 6 Preview Page
Assessment of High Temperature Impacts on Early Growth of Garlic Plant (Allium sativum L.) through Monitoring of Photosystem II Activities

광계II 활성 분석을 통한 마늘의 생육초기 고온 스트레스의 영향 평가

Soonja Oh1
,
Kyung Hwan Moon1
,
Seok Chan Koh2*
오 순자1
,
문 경환1
,
고 석찬2*
1Agricultural Research Institute for Climate Change, National Institute of Horticultural and Herbal Science
2Department of Biology, Jeju National University
1농촌진흥청 국립원예특작과학원 온난화대응농업연구소
2제주대학교 생물학과
*교신저자. *Corresponding Author. 
31 December 2015. pp. 829-838
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Abstract

Garlic (Allium sativum L.), one of the oldest cultivated crops, is the most widely used Allium species belonging to the family Lilliaceae. In this study, growth characteristics, photosystem II activity, and antioxidative enzyme activity were investigated in five temperatures (10-30°C) during early growth stage of garlic to determine the optimum temperature for cultivation and assess the effects of high temperature on early growth of garlic. Vegetative growth (e.g., shoot height, number of leaves) of garlic plants was greater in the temperature ranges of 15-25°C. However, dry weight (of shoot, bulb, and total plant) of garlic was significantly greater at 20°C, compared to either below or above 20°C. Fv/Fo and Fv/Fm values were highest at 15-20°C, and decreased above 25°C. The chlorophyll a fluorescence induction OKJIP transient was also considerably affected by high temperature; the fluorescence yields Fi and FP decreased considerably above 25°C, with the increase of Fk and Wk. Activities of catalase and superoxide dismutase in leaves and peroxidase in roots were high in 20-25°C, and decreased significantly in 30°C. These results indicate that a growth temperature of 30°C inhibits early growth of garlic and that it is desirable to culture garlic plants near 20°C. Fluorescence parameters such a Fv/Fo, Fv/Fm, Fk, ETo/CSm, and PIabs were significantly correlated with dry weight of whole garlic plants (p < 0.01), indicating that these fluorescence parameters can be used for early assessment of high temperature effects even though the damage to the plant is not very severe.

Keywords
antioxidative enzyme
chlorophyll a fluorescence parameter
early growth
OKJIP transient

본 연구는 마늘의 초기생장에 미치는 온도의 영향을 살펴보기 위하여 맹아가 출현된 마늘의 인편을 온도를 달리하여 재배하고, 마늘의 생육특성, 광계II 활성과 항산화효소의 발현 양상을 살펴보았다. 마늘의 생육은 15-25°C 조건에서 양호하여 지상부의 생장과 엽수가 빠르게 발달하였다. 특히, 지상부와 인경의 건중량과 전체 건중량이 20°C에서 가장 높았으며, 그보다 높은 온도에서는 지상부, 인경과 뿌리의 건중량이, 그리고 낮은 온도에서는 지상부의 건중량이 크게 감소하였다. Fv/Fo와 Fv/Fm 또한 15-20°C에서 높았으며 그 이상의 온도에서 크게 감소하였다. OKJIP 곡선의 패턴에서도 15-20°C에서 Fi, Fp가 다소 증가하고, 25°C 이상의 온도에서는 점차 감소하였으며, Fk와 Wk는 30°C에서 뚜렷하게 증가하였다. 그리고, 항산화효소들 중에 잎의 catalase와 superoxide dismutase, 그리고 뿌리의 peroxidase는 20-25°C에서 높고 30°C에서 크게 낮았다. 이러한 결과는 30°C 조건이 마늘의 초기생장에 있어 스트레스 요인으로 작용하고 있으며, 20°C 전후에서 재배하는 것이 바람직함을 나타내 주고 있다. 그리고, Fv/Fo, Fv/Fm, Fk, ETo/CSm, PIabs 등의 엽록소형광 변수들은 마늘의 전체 건중량과 의미있는 상관을 보여, 마늘 생산성을 사전에 예측하는데 유용한 변수로 활용될 수 있을 것으로 보인다.

키워드
항산화효소
엽록소형광 변수
초기생장
OKJIP곡선
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :6
  • Pages :829-838
  • Received Date : 2015-05-04
  • Revised Date : 2015-06-22
  • Accepted Date : 2015-06-22
  • DOI :https://doi.org/10.7235/hort.2015.15078
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