Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

Determining seed longevity and optimum storage conditions is essential for long-term seed storage. However, the longevity and optimal storage conditions of Pulsatilla dahurica are unknown. Here, P. dahurica seeds collected in Korea were stored under dry conditions at −20°C, 4°C, and 25°C immediately after collection. After 1, 2, 3, 6, and 12 months, the viability and germinability were measured. After 12 months, the seeds were subjected to saturated salt accelerated aging (SSAA) for additional aging, and the viability and germinability according to the storage temperature were confirmed. P. dahurica seeds did not lose viability or germinability, even after storage for 12 months at all temperatures; rather the germination index increased, resulting in fast and uniform germination. Differences in aging for storage at the three temperatures, which were not observed during actual storage, were identified using SSAA. Seeds stored at 25°C showed the lowest germinability and viability after accelerated aging. Notably, seeds stored at 4°C showed the highest germinability with a slower deterioration rate compared to those stored at −20°C. Collectively, P. dahurica seeds maintained germinability without a reduction in longevity at a wide temperature range during short- term storage (≤1 year), whereas for long-term storage, the optimum storage temperature was 4°C.