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2016 Vol.34, Issue 5 Preview Page
Characterization of Sources of Resistance to Bacterial Spot in Capsicum Peppers

고추 세균성점무늬병 저항성 유전자원과 그 주요 특성

Si–Eun Byeon1
,
Alebel Mekuriaw Abebe1
,
Yoon–Hyuk Jegal1
,
Khin Pa Pa Wai1
,
Muhammad Irfan Siddique1
,
Hwang-Sung Mo1
,
Hee Ju Yoo1
,
Kil–Su Jang2
,
Ji–Eun Hwang2
,
Su–Gyeong Jeon2
,
Su-Heon Lee3
,
Byung–Soo Kim1
변 시은1
,
Abebe Alebel Mekuriaw1
,
제갈 윤혁1
,
Wai Khin Pa Pa1
,
Siddique Muhammad Irfan1
,
모 황성1
,
유 희주1
,
장 길수2
,
황 지은2
,
전 수경2
,
이 수헌3
,
김 병수1
1Department of Horticulture, Kyungpook National University
2Yeongyang Pepper Experiment Station, Gyeongsangbuk–do Agricultural Research & Extension Services
3School of Applied Biosciences, Kyungpook National University
1경북대학교 원예학과
2영양고추시험장
3경북대학교 응용생명과학부
*교신저자. *Corresponding Author. 
31 October 2016. pp. 779-789
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Abstract

A total of 33 accessions of pepper (Capsicum spp.), including previously reported and newly discovered sources of resistance to bacterial spot caused by Xanthomonas euvesicatoria , were evaluated for their resistance to bacterial spot. The selected accessions were then grown and their horticultural characteristics were recorded. In a test for hypersensitive resistance (HR) to four races (P1, P3, P7, P8) of the pathogen found in Korea, KC00939 and Chilbok No.2, which carry the Bs2 gene, exhibited a hypersensitive response to all four races, as expected. Chilbok No.3, which carries the Bs3 gene, showed a hypersensitive reaction to race 1 and 7, as expected. KC00939 exhibited a high ASTA color value and tolerance to multiple infections from a viral complex of Cucumber mosaic virus (CMV) and Broad bean wilt virus (BBWV). Thus, this accession represents a promising genetic resource for breeding cultivars with multiple disease resistance and strong red coloration. KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127, KC01704, and KC00995 did not exhibit hypersensitivity but showed a high level of general resistance when evaluated by spray inoculation. KC01617, KC01760, KC01779, KC01137, KC01704, and KC01777 are newly identified sources of resistance to bacterial spot. The previously and newly identified sources of resistance to bacterial spot evaluated in this study, including information about their resistance to CMV and BBWV complex in the field, the contents of pungent and sweet taste components, and the color values of dry fruits, will be useful for breeding pepper cultivars with resistance to bacterial spot.

Keywords
Capsicum spp.
general resistance
hypersensitive resistance
race
Xanthomonas euvesicatoria

국내외에서 보고된 저항성 유전자원과 새로 찾은 자원을 포함한 고추 유전자원 총 33점에 세균성점무늬병원균(X.euvesicatoria)을 접종하여 과민반응형 저항성과 일반 저항성을 검정하고, 선발한 자원에 대하여 원예적 특성을 조사하였다. 국내에 보고된 4종의 race(1, 3, 7, 8)에 대한 과민반응형 저항성을 판별한 결과, Bs2 유전자를 보유한 KC00939와 Chilbok No.2는 예상대로 4종의 race에 과민형 반응을 나타내었다. Bs3 유전자를 보유한 Chilbok No.3는 예상대로 race 1과 7에 과민형 반응을 보였다. KC00939는 CMV와 BBWV의 복합 감염에 강한 저항성을 보이고 색소함량이 매우 높아 고색도 복합저항성 육종소재로서의 그 가치가 높았다. 이와 더불어 KC01327, KC01617, KC01015, KC01760, KC01779, KC01137, KC01328, KC01006, KC00127(PI369994), KC01704, KC00995, KC00131(PI369998) 및 KC01777이 높은 수준의 일반 저항성을 나타내었다. 이들 중 KC01617, KC01760, KC01779, KC01137, KC01704, KC01777은 이번 연구에서 세균성점무늬병 저항성으로 찾은 자원이다. 이들 세균성점무늬병 저항성 유전자원의 CMV와 BBWV의 복합 감염에 대한 저항성, 과실특성, 과실의 신미, 감미 및 색소함량(ASTA)을 분석한 결과는 세균성점무늬병 저항성 육종에 유용하게 활용될 것으로 기대된다 .

키워드
Capsicum spp.
일반 저항성
과민반응형 저항성
레이스
Xanthomonas euvesicatoria
References
1
Attuquayefio VK, Bukle KA (1987) Rapid sample preparation method for HPLC analysis of capsaicinoids in Capsicum fruits and oleoresins. J Agric Food Chem 35:777–779. doi:10.1021/jf00077a032
2
Cook AA, Guevara YG (1984) Hypersensitivity in Capsicum chacoense to race 1 of the bacterial spot pathogen of pepper. Plant Dis 68:329–330. Doi:10.1094/PD-68-329
3
Cook AA, Stall RE (1963) Inheritance of resistance in pepper to bacterial spot. Phytopathology 53:1060–1062
4
Cook AA, Stall RE (1969) Differentiation of pathotypes among isolates of Xanthomonas vesicatoria. Plant Dis Rep 53:617–619
5
EPPO (2013) PM 7/110 (1) Xanthomonas spp. (Xanthomonas euvesicatoria, Xannthomonas gardneri , Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper. EPPO Bull 43:7–20. doi:10.1111/epp.12018
6
Hibberd AM, Stall RE, Bassett MJ (1987) Different phenotypes associated with incompatible races and resistance genes in bacterial spot disease of pepper. Plant Dis 71:1075–1078. doi:10.1094/PD-71-1075
7
Iwai K, Suzuki T, Fujiwake H (1979) Formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues, in Capsicum annuum var. annuum cv. Karayatsubusa at different growth stages after flowering. Agric Biol Chem 43: 2493–2498. doi: 10.1271/bbb1961.43.2493
8
Jones JB, Lacy GH, Bouzar H, Stall RE, Schaad NW (2004) Reclassification of the Xathomonads associated with bacterial spot disease of tomato and pepper. System Appl Microbiol 27:755–762. doi: 10.1078/0723202042369884
9
Jones JB, Minsavage GV, Roberts PD, Johnson RR, Kousik CS (2002) A non–hypersensitive resistance in pepper to the bacterial spot pathogen is associated with two recessive genes. Phytopathology 92:273–277. doi: 10.1094/PHYTO.2002.92.3.273
10
Jones JB, Stall RE, Bouzar H (1998) Diversity among xanthomonads pathogenic on pepper and tomato. Ann Rev Phytopathol 36:41– 58. doi:10.1146/annurev.phyto.36.1.41
11
KATS (2013) Red pepper ground. https://standard.go.kr/KSCI/standardIntro/getStandardSearchListResult.do Accessed 12 January 2015
12
Kim BS, Hartmann RW (1985) Inheritance of a gene (Bs3) conferring hypersensitive resistance to Xanthomonas campestris pv. vesicatoria in pepper (Capsicum annuum). Plant Dis 69:233–235
13
Kim BS, Kim YC, Shin KS, Kim JH (2007) Near–isogenic lines for genes conferring hypersensitive resistance to bacterial spot in chili pepper. Plant Pathol J 23:155–166. doi: 10.5423/PPJ.2007.23.3.155
14
Kim BS, Kwon YS, Hur JM (1990) Differentiation and distribution of pathotypes of Xanthomonas campestris pv. vesicatoria pathogenic on pepper in Korea. Korean J Plant Pathol 6:245–249
15
Kim BS, Kwon YS, Shon EY (1991) Inheritance of resistance to bacterial spot and to Phytophthora blight in peppers. Agric Res Bull Kyungpook Natl Univ 9:127–133
16
Kim BS, Souvinmonh B, Son K, Ahn JH, Lee SM (2009) New additions to sources of resistance to bacterial spot and field performance of HR gene NILs in Capsicum pepper. Hort Environ Biotechnol 50:566–570
17
Kim CH, Ryu SH, Lee MJ, Baek JW, Hwang HC, Moon GS (2004) Characteristics of red pepper (Capsicum annuum L.) powder using N2–circulated low temperature drying method. Korean J Food Sci Technol 36:25–31
18
Kousik CS, Ritchie DE (1999) Development of bacterial spot on near-isogenic lines of bell pepper carrying gene pyramids composed of defeated major resistance genes. Phytopathology 89:1066–1072. doi: 10.1094/PHYTO.1999.89.11.1066
19
Lee SD, Cho YS (1996) Copper resistance and race distribution of Xanthomonas campestris pv. vesicatoria on peper in Korea. Korean J Plant Pathol 12:150–155
20
Lee SH, Lee JB, Kim SM, Choi HS, Park JW, Lee JS, Lee KW, Moon JS (2004) The incidence and distribution of viral diseases in pepper by cultivation types. Res Plant Dis 10:231–240. doi:10.5423/RPD.2004.10.4.231
21
Luo XJ, Peng J, Li YJ (2011) Recent advances in the study on capsaicinoids and capsinoids. European J Pharmacol 650:1–7. doi:10.1016/j.ejphar.2010.09.074
22
MAFRA (2015) Status of the greenhouse for facility vegetable and the production results of vegetables. The Ministry of Agriculture, Food and Rural Affairs, Sejong, Korea. p 11
23
Minsavage GV, Dahlbeck D, Whalen MC, Kearney B, Bonas U, Staskawicz BJ, Stall RE (1990) Gene–For–Gene relationships specifying disease resistance in Xanthomonas Campestris pv. vesicatoria – pepper interactions. Mol Plant–Microb Interact 3:41–47. doi: 10.1094/MPMI-3-041
24
Mo HS, Jang KS, Hwang JE, Jeon SG, Kim BS (2015) Horticultural and chemical quality characterization of accessions selected from four species of Capsicum. Hort Environ Biotechnol 56:54–66. doi:10.1007/s13580–015–0078–0
25
Pae DH, Yoon JY, Lee, JM (1994) Pathotype distribution of Xanthomonas campestris pv. vesicatoria on pepper (Capsicum annuum) in Korea and cross-protection between the pathotypes. J Korean Soc Hort Sci 35:126-130
26
Poulos JM, Reifschneider FJB, Coffman WR (1991) Heritability and gain from for quantitative resistance to Xanthomonas campestris pv. vesicatoria in Capsicum annuum L. Euphytica 56:161–167. doi: 10.1007/BF00042060
27
Sahin F, Miller SA (1998) Resistance in Capsicum pubescens to Xanthomonas campestris pv. vesicatoria pepper race 6. Plant Dis 82:794–799. doi: 10.1094/PDIS.1998.82.7.794
28
Schaad NW, Jones JB, Chun W (2001) Laboratory guide for identification of plant pathogenic bacteria. In St. Paul, Minnesota: APS Press, Ed 3, USA, pp 1–15
29
Son SM, Lee JM, Oh MS (1995) A comparative study of nutrients and taste components in Korean and imported red peppers. Korean J Nutr 28:53–60
30
Sowell G Jr (1960) Bacterial spot resistance of introduced peppers. Plant Dis Rep 44:587–590
31
Stall RE, Jones JB, Minsavage GV (2009) Durability of rsistance in tomato and pepper to Xanthomonads causing bacterial spot. Annu Rev Phytopathol 47:265–284. doi:10.1146/annurev–phyto–080508–081752
32
Tran NH, Kim BS (2007) Search for new sources of resistance to bacterial spot (Xanthomonas campestris pv. vesicatoria) in Capsicum pepper. Acta Hort 760:323–328. doi:10.17660/ActaHortic.2007.760.44
33
Wai KPP, Siddique MI, Mo HS, Yoo HJ, Byeon SE, Jegal YH, Abebe AM (2015) Pathotypes of bacterial spot pathogen infecting Capsicum peppers in Korea. Plant Pathol J 31:428–432. doi:10.5423/PPJ.NT.05.2015.0074
34
Yang EY, Cho MC, Lee WM, Kim S, Park DK, Yoon MK (2012) Preparation for the future of pepper industry. RDA Interrobang 79:1–20
35
YPES (2005) Yeongyang Pepper Experiment Station Policy Proposal. Available via http://pepper.gba.go.kr/page.htm?mcode= 1295661666 Accessed 12 January 2015
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 34
  • No :5
  • Pages :779-789
  • Received Date : 2016-01-14
  • Revised Date : 2016-05-09
  • Accepted Date : 2016-08-25
  • DOI :https://doi.org/10.12972/kjhst.20160082
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