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2017 Vol.35, Issue 6 Preview Page
Prenylflavonoid Contents of Five Epimedii Herba and Their Antioxidant Activity in PC12 Cells

음양곽 5종의 프레닐플라보노이드 함량에 따른 항산화 활성 및 산화적 스트레스에 대한 PC12 세포 보호효과

Miran Jang1
,
Cheon-Ho Jo2
,
Ji Young Kim1*
장 미란1
,
조 천호2
,
김 건희1*
1Plant Resources Research Institute, Duksung Women’s University
2New Hazardous Substance Team, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety
1덕성여자대학교 식물자원연구소
2식품의약품안전처 신종물질유해팀
*교신저자. *Corresponding Author. 
31 December 2017. pp. 793-804
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Abstract

This study was conducted to comparatively evaluate antioxidant activity of five species of Epimedium genus (Epimedium koreanum , E. brevicornum , E. pubescens , E. sagittatum and E. wushanense ) and their protective effects on neuronal PC12 cells. The contents of individual penylflavonoids in five Epimedium species were also examined using HPLC/DAD. Five Epimedium plants were composed of icariin, icariside I, II, icaritin and epimedin A-C. Icariin was most predominant among all detected prenylflavonoids at all species studied. Icariin contents were in the order of E. koreanum (3.66 mg·g-1 DW) > E. wushanense (1.98 mg·g-1 DW) >=E. sagittatum (1.87 mg∙g-1 DW) > E. pubescens (1.60 mg∙g-1 DW) > E. brevicornum (0.75 mg∙g-1 DW). The highest antioxidant values studied via ORAC, DPPH, ABTS and FRAP assay were found in E. koreanum extract among the species. In addition, the neuroprotective effect of E. koreanum extract was strongest among studied via MTT, LDH and DCH-DA assay using H2O2-damaged PC12 cells species. All tested Epimedium plants extract showed neuronal cell protective effects in a dose-dependent manner. These results indicated that Epimedium plants extract has potent antioxidant and neuronal protective effects and E. koreanum extract had the highest activities among tested five species especially.

Keywords
Epimedium brevicornum
Epimedium koreanum
Epimedium pubescens
Epimedium sagittatum
Epimedium wushanense
Neuronal protective effects

본 연구는 5종의 음양곽에 대하여 프레닐플라보노이드 함량 분석과 항산화능 및 신경세포 보호활성을 측정하였다. 실험에 사용된 음양곽은 삼지구엽초(Epimedium koreanum), 중국음양곽(E. brevicornum), 유모음양곽(E. pubescens), 전엽음양곽(E. sagittatum) 및 무산음양곽(E. wushanense)으로 HPLC/DAD를 이용하여 분석한 결과 프레닐플라보노이드인 이카린, 이카리 사이드 I 및 II, 이카리틴, 에피메딘 A-C가 5종의 음양곽에 함유되어 있었으며, 플라보노이드 배당체인 히페린이 삼지구엽초 에만 함유되어 있었다. 이카린은 모든 음양곽에서 가장 많이 함유되어 있었으며 (0.08–0.37%, w:w) 그 순서는 삼지구엽초(3.66 mg∙g-1 DW) > 무산음양곽(1.98mg∙g-1 DW) ≧ 전엽음양곽(1.87mg∙g-1 DW) > 유모음양곽(1.60mg∙g-1 DW) > 중국음양곽 (0.75mg∙g-1 DW)으로 나타났다. ORAC, DPPH, ABTS 및 FRAP 법을 활용하여 삼지구엽초의 항산화 활성을 측정한 결과 모 든 시험법에서 삼지구엽초의 항산화 활성이 가장 뛰어난 것으로 확인되었다. 또한, 과산화수소로 산화적 손상을 유도한 PC12 세포에 대한 MTT, LDH 및 DCA-DA 법 결과 역시 삼지구엽초의 세포보호효과가 가장 뛰어났으며 5종의 음양곽 모두 농도 의존적으로 세포 보호 활성이 나타났다. 본 연구를 통하여 5종의 음양곽의 주요 활성 성분 및 그에 따른 생리활성의 차이를 확 인하였으며, 특히, 삼지구엽초의 우수한 항산화 활성 및 신경세포 보호효과가 검증되었다 .

키워드
Epimedium brevicornum
Epimedium koreanum
Epimedium pubescens
Epimedium sagittatum
Epimedium wushanense
항산화
신경보호효
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 35
  • No :6
  • Pages :793-804
  • DOI :https://doi.org/10.12972/kjhst.20170083
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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