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2014 Vol.32, Issue 3 Preview Page
Major Metabolites Involved in Skin Blackening of ‘Niitaka’ Pear Stored under Cold Temperature

신고 배 저온 저장 중 발생하는 과피 흑변에 관여하는 주요 대사체

Lee Eun Jin12*
이 은진12*
1Department of Horticultural Science and Biotechnology, Seoul National University
2Research Institute of Agriculture and Life Sciences, Seoul National University
1서울대학교 원예생명공학과
2서울대학교 농생명과학연구원
*교신저자. *Corresponding Author. 
30 June 2014. pp. 359-365
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Abstract

Oriental pear (Pyrus communis L. cv ‘Niitaka’) was stored at 0°C for 5 months and major metabolites involved in blackening of the peel were analyzed by untargeted GC-MS and targeted HPLC methods. In this study, peels of sound and skin-blackened pears were analyzed and compared. Skin-blackened fruit was clearly characterized by a distinctive pattern in changes which included a decrease of malic acid, succinic acid, and ascorbic acid, while an increase of fumaric acid, threonine, and gluconic acid, which indicated both reduced metabolic activity and anti-oxidative capacity of the cells. Chlorogenic acid was a major phenolic compound and the peel of sound fruit showed high levels of free phenolic compounds compared than the peel of skin-blackened fruit which are believed to be related to oxidation of phenolics in skin-blackened tissue. The changes or profiling of major metabolites by targeted or untargeted analysis method could become a useful tool for understanding physiology, disorder mechanism, and identifying metabolic networks connecting primary and secondary metabolism in postharvest research.

Keywords
discoloration
GC-MS
physiological disorder
storage

본 연구는 신고 배 저온 저장 중 발생하는 과피 흑변에 관여하는 주요 대사체를 untargeted GC-MS 그리고 targeted HPLC로 알아보고자 하였다. GC-MS 분석 결과 아미노산, 유기산, 당 관련 총 40개의 대사체를 정성할 수 있었고 건전과는 과피 흑변과에 비해서 전반적으로 높은 대사체 함량을 보였다. 특히 serine, malic acid, succinic acid가 건전과에서 높게 나타난 반면 과피 흑변과에서는 threonine, fumaric acid, gluconic acid 함량이 높았다. 개개의 phenolic compounds 분석 결과 건전과에서 높은 phenolic compounds 함량을 보였다. 또한 항산화 능력과 깊은 관련이 있는 ascorbic acid가 과피 흑변 과에서 낮게 나타났다. 이상의 결과로 보아 세포 내 비정상적인 호흡 및 에너지 대사과정, 항산화 능력 결여가 신고 배 과피 흑변에 관여하는 주요 대사 과정임을 확인할 수 있었다.

키워드
변색
질량분석
생리장해*
저장
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :3
  • Pages :359-365
  • Received Date : 2013-12-08
  • Revised Date : 2013-12-27
  • Accepted Date : 2014-02-05
  • DOI :https://doi.org/10.7235/hort.2014.13191
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