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2014 Vol.32, Issue 5 Preview Page
Changes in Cell Ca2+ Distribution in Loquat Leaves and Its Effects on Cold Tolerance
Guohua Zheng1
,
Dongming Pan1*
,
Xianqian Niu2
,
Hanwen Wu3
,
Jinbiao Zhang4
1Horticulture College, Fujian Agricultural and Forestry University
2Fujian Science Institute of Tropical Crops
3EH Graham Centre for Agricultural Innovation (NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute
4School of Life Sciences, Fujian Agriculture and Forestry University
*교신저자. *Corresponding Author. 
31 October 2014. pp. 607-613
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Abstract

Calcium has been associated with improved cold tolerance in many crops. The aim of this study was to investigate the changes in leaf cell Ca2+ distribution and cell organelle ultrastructure of loquat (Eriobotrya japonica Lindl.) plants in response to cold stress at -3°C, using transmission electron microscopy (TEM). Two loquat accessions, Zaozhong 6 (a commercial cultivar) and oakleaf loquat (a wild relative) were used. Cold tolerance, as measured by leaf browning rate, was higher in oakleaf plants, and calcium treatment improved cold tolerance in both species. Cold stress first induced inward transport of Ca+2 from the intracellular space. Then, the imported Ca2+ was aggregated around the chloroplast membrane, finally entering the chloroplast. This pattern of Ca2+ distribution in leaf cells occurred earlier in Zaozhong 6 than in the wild loquat. With increasing time of cold exposure, the chloroplast membranes of Zaozhong 6 leaves were damaged, blurred and even disappeared, while those of wild oakleaf loquat leaves maintained their structure longer. In Zaozhong 6, cold stress induced a clear cavity between poorly structured granal thylakoids and vesicles appearing inside the chloroplast, while in oakleaf leaves cold stress had little effect on the ultrastructure of chloroplasts (although chloroplast membranes looked blurred). Loquat leaves accumulated free calcium ions around chloroplasts in response to cold stress, with earlier calcium accumulation occurring in the cold-sensitive cultivar Zaozhong 6 than in wild oakleaf loquat. These results demonstrate that these two loquat species have differences in both cold tolerance and calcium accumulation dynamics.

Keywords
cold stress
injury
low temperature treatment
subcellular localization
ultrastructure

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :5
  • Pages :607-613
  • Received Date : 2013-01-09
  • Revised Date : 2014-04-26
  • Accepted Date : 2014-06-08
  • DOI :https://doi.org/10.7235/hort.2014.13009
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