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2015 Vol.33, Issue 6 Preview Page
Improvement of Chromosome Doubling Efficiency in Cymbidium Hybrids by Colchicine and Oryzalin Treatment

Colchicine과 oryzalin 처리에 의한 Cymbidium hybrids의 기내 배수화 효율 증진

Sook-Hyun Hwang1,2*
,
Mi-Seon Kim1
,
So-Young Park2
황 숙현1,2*
,
김 미선1
,
박 소영2
1Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration
2Department of Horticultural Science, Chungbuk National University
1국립원예특작과학원 화훼과
2충북대학교 축산･원예･식품공학부 원예학전공
*교신저자. *Corresponding Author. 
31 December 2015. pp. 900-910
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Abstract

This study aimed to investigate the efficiency of colchicine and oryzalin in inducing polyploidy in two Cymbidium hybrids [Showgirl ‘Silky’ and Mystery Island ‘Silk Road’ (Silk Road-4)]. Colchicine was used at concentrations ranging from 50 to 500 mg･L-1, with treatments lasting 1 to 3 weeks. Oryzalin was used at concentrations ranging from 3 to 20 mg･L-1, with treatments lasting 3 to 6 days or 1 to 3 weeks. The survival rate of PLBs was better in colchicine than in oryzalin solutions. The ploidy levels were screened using flow cytometry. In C. Showgirl ‘Silky’, the highest chromosome doubling efficiencies were obtained with the 1-week treatment in 50 mg･L-1 colchicine (60%) and the 2-week treatment in 5 mg･L-1 oryzalin (46.7%). In C. Mystery Island ‘Silk Road’ (Silk Road-4), the highest chromosome doubling efficiencies were obtained with the 1-week treatment in 50 mg･L-1 colchicine (16.7%) and the 3-day treatment in 10 mg･L-1 oryzalin (6.7%). Colchicine was more efficient than oryzalin in terms of polyploidy induction. Furthermore, pre-treatment, which entailed poking 10 times with forceps, improved the efficiency of chromosome doubling.

Keywords
chromosome counting
flow cytometry
guard cell
hexaploid
polyploid induction
protocorm-like bodies

본 연구는 우리나라 주요 난과식물 중 하나인 심비디움의 배수체 육종을 위한 기초자료를 얻고자 실시되었으며, 기내 심비디움의 PLBs에 colchicine과 oryzalin을 처리방법과 농도, 기간을 다양하게 하여 배수체 유도에 최적의 조건을 찾고자 수행하였다. Colchicine은 50, 100, 300, 500mg･L-1 농도로 1, 2, 3주간 처리하였고 oryzalin은 3, 5, 10, 20mg･L-1 농도로 1, 2, 3주 또는 3, 6, 9일간 처리하였는데 oryzalin에 비해 colchicine을 처리했을 때 생존율이 높게 나타났다. Flow cytometry를 이용하여 배수성을 검정해본 결과 Cymbidium Showgirl ‘Silky’의 경우, colchicine은 50mg･L-1 농도로 1주간 처리 시 6배체 획득효율이 60%, oryzalin은 5mg･L-1 농도로 2주간 처리 시 6배체 획득효율이 46.7%로 가장 높게 나타났다. Cymbidium Mystery Island ‘Silk Road’(Silk Road-4)의 경우, colchicine은 50mg･L-1 농도로 1주간 처리 시 6배체 획득효율이 16.7%, oryzalin은 10mg･L-1 농도로 3일간 처리 시 6배체 획득효율이 6.7%로 가장 높게 나타났다. 즉, 두 hybrid 모두 oryzalin보다 colchicine을 처리했을 때 염색체 배가에 더 효율적이었다. 또한 처리 전 PLBs를 뾰족한 핀셋으로 10회 찌르는 방법을 통해 염색체 배가 효율을 향상시킬 수 있는 것으로 나타났다.

키워드
염색체 수
유세포분석
공변세포
6배체
배수체 유도
상구체
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 33
  • No :6
  • Pages :900-910
  • Received Date : 2015-04-01
  • Revised Date : 2015-07-23
  • Accepted Date : 2015-08-04
  • DOI :https://doi.org/10.7235/hort.2015.15063
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