Effects of Medium Components and Composition on Mass Propagation of Arachniodes aristata (G. Forst.) Tindale

Ju Sung Cho; Ji Hyun Han; Cheol Hee Lee

doi:10.12972/kjhst.20170014

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2017 Vol.35, Issue 1 Preview Page Next Page

Effects of Medium Components and Composition on Mass Propagation of Arachniodes aristata (G. Forst.) Tindale 가는쇠고사리의 대량번식에 미치는 배지구성물질과 배양토의 영향

28 February 2017. pp. 131-141

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Abstract

This study was conducted to investigate in vitro mass propagation methods suitable for each growth stage of A. aristata (G. Forst.) Tindale, from spore germination to sporophyte formation. Among spores germinated in 1/8 - 1 × MS medium and Knop medium, Knop medium yielded the highest germination percentage (87.1%). We cultured prothalli obtained from germinating spores for 8 weeks on media with different concentrations of sucrose and active carbon, as well as different concentrations and ratios of nitrogen, to select a suitable growth medium. A. aristata (G. Forst.) Tindale prothalli grew most actively in MS medium with 3% sucrose and 20 : 40 mM of NH4Cl and KNO3 (total concentration of 60 mM). We investigated sporophyte formation according to soil type, finding that bedding soil mixed with perlite at a 2 : 1(v / v) ratio yielded the highest number of sporophytes per pot (73.8 / 7.5 × 7.5 cm pot). By contrast, when peat moss was used alone or mixed with other substrates, prothallus development and sporophyte formation were suppressed. Therefore, the most effective propagation method for A. aristata (G. Forst.) Tindale is to grow prothalli in MS medium and to induce sporophyte formation in a mixture of bedding soil and perlite (v / v = 2 : 1).

Keywords

Dryopteridaceae

fern

in vitro propagation

prothallus

sporophyte

본 연구는 면마과 가는쇠고사리[A. aristata (G. Forst.) Tindale]의 각 생육단계에 적합한 기내 대량증식법을 구명하기 위해 수 행되었다. 포자를 1 / 8 1배로 조절한 MS 배지와 Knop 배지에 파종한 결과, Knop 배지에서 최종 발아율이 87.1%로 가장 높 았다. 또한 전엽체를 배지의 종류, sucrose와 활성탄의 농도, 질소급원의 농도 및 비율을 다르게 처리하여 8주간 배양하였다. 실험의 결과, 가는쇠고사리의 전엽체는 sucrose 3%의 MS배지에서 NH4Cl과 KNO3의 비율을 20 : 40mM로 하여 총 농도를 60mM로 조절한 배지에서 증식이 가장 왕성하였다. 토양 종류에 따른 포자체 형성을 관찰한 결과, 상토:펄라이트를 2 : 1(v / v) 로 혼용하였을 때, 단위면적 당 발생한 포자체의 수가 73.8개 / 7.5 × 7.5cm로 가장 많았다. 반면 피트모스를 단용 및 혼용처리 한 조건에서는 전엽체 발달 및 포자체 형성이 억제되었다. 따라서 가는쇠고사리는 MS 배지에서 전엽체를 증식시킨 다음 상 토 : 펄라이트(v / v = 2 : 1) 혼합토양에서 포자체 형성을 유도하는 것이 효과적인 증식법으로 생각된다 .

키워드

면마과

양치식물

기내대량증식

전엽체

포자체

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :한국원예학회
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 35
No :1
Pages :131-141
Received Date : 2016-02-23
Revised Date : 2016-08-07
Accepted Date : 2016-10-09
DOI :https://doi.org/10.12972/kjhst.20170014

Horticultural Science and Technology 원예과학기술지 ISSN:1226-8763(Print) 2465-8588(Online)

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