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2020 Vol.38, Issue 4 Preview Page

Research Article

Development of an Efficient Screening Method for Resistance of Chinese Cabbage Cultivars to Black Rot Disease Caused by Xanthomonas campestris pv. campestris

Xanthomonas campestris pv. campestris에 의한 배추 검은썩음병의 효율적인 저항성 검정법 개발

Soo Min Lee12
,
Yong Ho Choi1
,
Heung Tae Kim2
,
Gyung Ja Choi13*
이 수민12
,
최 용호1
,
김 흥태2
,
최 경자13*
1Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology
2Department of Plant Medicine, Chungbuk National University
3Department of Medicinal Chemistry and Pharmacology, University Science and Technology
1한국화학연구원 친환경신물질연구센터
2충북대학교 식물의학과
3과학기술연합대학원대학교 의약화학 및 약리생물학과
* Corresponding Author
31 August 2020. pp. 547-558
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Abstract

Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most serious diseases of crucifers world-wide. To establish the efficient screening methods for resistant Chinese cabbage to Xcc, the resistance degree of 88 commercial cultivars of Chinese cabbage to Xcc KACC 10377 was examined. For further study, we selected six Chinese cabbage cultivars (resistant: Mansumugang, Woldongcheonha, CR Anshim; susceptible: Bulam3ho, Chunkwang, CR Nongshim) showing different disease response to the Xcc KACC 10377. According to growth stages of Chinese cabbage seedlings, inoculation density, and incubation temperature and period after inoculation in a dew chamber, development of black rot on the six cultivars was investigated. From the results, we suggest the following method for selection of Chinese cabbage seedlings that are resistant to Xcc. The efficient method is to inoculate 10-day-old Chinese cabbage seedlings with an cell suspension of Xcc at a concentration of 1.0 × 108 cfu/mL using the spray method and to incubate the inoculated plants in a dew chamber at 30°C for 48 hours and then transfer to a growth chamber at 25°C with 80% RH under a 12 hour light/dark cycle. Disease severity of the plant can be measured six days after inoculation.

Keywords
bacteria
breeding
condition
plant disease
vegetable

Xanthomonas campestris pv. campestris(Xcc)에 의한 검은썩음병은 전세계적으로 배추과 작물에 발생하는 가장 심각한 병 중 하나이다. 본 연구는 배추 검은썩음병의 효과적인 병리검정법을 확립하기 위하여, 시판 배추 품종 88개를 구입하고 이들 품종의 Xcc KACC 10377에 대한 저항성을 조사하여 저항성 정도가 다른 6개의 배추 품종(저항성: 만수무강, 월동천하, CR안심; 감수성: 불암3호, 춘광, CR농심)을 선발하였다. 선발한 6개 배추 품종을 사용하여 배추 유묘의 생육 정도, Xcc 접종원 농도 그리고 병원균 접종 후 습실처리의 온도 및 기간에 따른 이들 품종들의 검은썩음병 발생을 조사하였다. 이들 결과로부터 배추 품종들의 검은썩음병에 대한 저항성 정도를 검정하기 위한 방법으로, 배추 종자를 파종하고 온실(25 ± 5°C)에서 10일 동안 재배한 배추 유묘에 1.0 × 108cfu/mL 농도로 조정한 Xcc 세균현탁액을 분무하여 접종하고, 접종한 식물을 30°C에서 48시간 동안 습실처리한 후에 생육상(25°C, 상대습도 80%)으로 이동하여 광을 조사하면서 6일 동안 재배한 후에 검은썩음병 발생을 조사하는 것을 제안하고자 한다.

키워드
세균
육종
조건
식물병
채소
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :4
  • Pages :547-558
  • Received Date : 2020-01-26
  • Revised Date : 2020-03-20
  • Accepted Date : 2020-06-07
  • DOI :https://doi.org/10.7235/HORT.20200051
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