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Effect of MS Medium Strength on the Sprouting Rate and Growth Characteristics in Meristem Culture of Strawberry ‘Seolhyang’

‘설향’ 딸기의 생장점 배양 시 MS 배지 농도에 따른 발아율 및 생육특성

Kim Hye Jin1
,
Lee Jong Nam1*
,
Lim Hak Tae2
,
Yeoung Young Rok3
김 혜진1
,
이 종남1*
,
임 학태2
,
용 영록3
1Highland Agriculture Research Center, National Institute of Crop Science, Rural Development Administration
2Department of Bio-Health Technology, Kangwon National University
3Department of Plant Science, Gangneung-Wonju National University
1국립식량과학원 고령지농업연구센터
2강원대학교 생명건강공학전공
3강릉원주대학교 식물생명과학과
*교신저자. *Corresponding Author. 
28 February 2014. pp. 100-104
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Abstract

This study was conducted to determine the optimal MS medium strength to improve sprouting rate of apical meristem of strawberry ‘Seolhyang’ in vitro. Strawberry apical meristems at size (0.2 mm to 0.3 mm) with leaf primordials were cultured on the MS media with four strength levels (1/4×, 1/3×, 1/2×, and 1×) and the sprouting rate and growth characteristics were evaluated after eight weeks after cultivation. Shoot rate of ‘Daewang’ apical meristems was 93.6% whereas ‘Seolhyang’ apical meristems were sprouted with 31.6% on 1× MS medium strength. Different sprouting rates were observed in ‘Seolhyang’ apical meristem with 31.6% in 1× medium, 75.0% in 1/2× medium, and 94.4% in 1/3× medium. The sprouting rate was improved with the decrease of medium strength, but the shoot rate in 1/4× medium decreased up to 54.5%. Shoot length was 0.9 cm in 1× medium, 1.2 cm in 1/2× medium, 1.6 cm in 1/3× medium, and 1.9 cm in 1/4× medium. Shoot length was longer as medium strength decreased and numbers of leaves and roots were not significant differences among the medium strengths. As a result, sprouting rate was highest and plant growth was best in 1/3× MS medium compared to the others.

Keywords
Daewang
Fragaria × ananassa
leaf primordial
optimal
shoot length

본 실험은 일계성 딸기 ‘설향’의 조직배양묘 생산 시 발아율 향상을 위해 MS 배지의 적정 농도를 구명하기 위하여 실시하였다. 딸기 생장점은 엽원기가 1매 붙은 0.2mm-0.3mm 크기로 적출하였으며, MS 배지 농도는 1/4, 1/3, 1/2, 1배 등 4 수준으로 실시하여 배양 8주 후 발아율 및 생육 특성을 조사하였다. 일계성 딸기 ‘설향’과 ‘대왕’의 생장점을 1배 농도의 MS 배지에 치상한 결과, ‘대왕’의 발아율은 93.6%인 반면에 ‘설향’의 발아율은 31.6%로 매우 낮았다. ‘설향’의 발아율을 향상시키기 위하여 MS 배지 농도별 실험한 결과, 1배 농도의 발아율은 31.6%, 1/2배 농도는 75.0%, 1/3배 농도는 94.4%로 배지 농도가 낮아질수록 발아율이 향상되었으나, 1/4배 농도에서 발아율이 54.5%로 낮아졌다. 생장점 배양 8주 후 MS 배지 농도별 생육을 비교한 결과, 초장은 1배 농도에서 0.9cm, 1/2배 농도에서 1.2cm, 1/3배 농도에서 1.6cm, 그리고 1/4배 농도에서 1.9cm로 배지 농도가 낮아질수록 초장이 길어졌으나, 잎수 및 뿌리수는 큰 차이를 보이지 않았다. 본 실험의 결과 일계성 딸기 ‘설향’의 생장점 배양 시 1/3배 MS 배지에서 발아율이 가장 높고, 생육이 우수하였다.

키워드
대왕
Fragaria × ananassa
엽원기
최적
초장
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :1
  • Pages :100-104
  • Received Date : 2013-06-07
  • Revised Date : 2013-07-26
  • Accepted Date : 2013-10-01
  • DOI :https://doi.org/10.7235/hort.2014.13089
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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