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A Database of Simple Sequence Repeat (SSR) Marker-Based DNA Profiles of Citrus and Related Cultivars and Germplasm

SSR Marker를 이용한 감귤속 품종 및 유전자원에 대한 DNA Profile Data Base 구축

Jee-Hwa Hong1
,
Chi-Won Chae2
,
Keun-Jin Choi3
,
Yong-Sham Kwon4*
홍 지화1
,
채 치원2
,
최 근진3
,
권 용삼4*
1Experiment Research Institute, National Agricultural Products Quality Management Service, Ministry for Agriculture, Food & Rural Affairs
2Jeju Office, Korea Seed & Variety Service, Ministry for Agriculture, Food & Rural Affairs
3Vegetable Research Center, National Institute of Horticultural & Herbal Science
4Department of Genetic Engineering, College of Natural Resources and Life Science, Dong-A University
1농림축산식품부 국립농산물품질관리원 시험연구소
2농림축산식품부 국립종자원 제주지원
3국립원예특작과학원 채소과
4동아대학교 생명자원과학대학 유전공학과
*교신저자. *Corresponding Author. 
28 February 2016. pp. 142-153
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Abstract

The present study investigated identification of cultivars through phylogenetic analysis of 108 Citrus varieties and related cultivars using simple sequence repeat (SSR) markers. Two hundred three SSR primer pairs were used to detect polymorphic markers among 8 Citrus cultivars consisting of 4 mandarins, 1 orange, 1 tangor, 1 tangelo, and 1 pumelo. Eighteen SSR primer pairs were reproducible and showed highly polymorphic alleles. These markers were applied to assess genetic variations of the 108 varieties. Each marker detected 5-14 alleles, with an average of 9.28. The polymorphism information content varied from 0.417 to 0.791 with an average of 0.706. Cluster analysis with SSR markers resulted in 13 major groups reflecting cultivar types and pedigree information. Twelve orange cultivars in the I-1st sub-cluster and 23 mandarin cultivars in the II-1st sub-cluster, respectively, were not discriminated using the SSR markers. This could be due to narrow genetic backgrounds originated through bud mutation or nucellars seedlings. The SSR profile database of Citrus cultivars will be useful as a tool for protection of plant breeders’ intellectual property rights in addition to assessing genetic diversity in Citrus cultivars and germplasms.

Keywords
cultivar identification
genetic relationship
mandarin
microsatellite
molecular marker

국내외에서 재배되고 있는 감귤속 식물 108 품종 및 유전자원과 SSR 마커를 활용하여 유전적 유사도 분석을 통한 품종식별 력 검정 등에 대한 연구를 수행하였다. 감귤 8품종을 203개의 SSR 마커로 검정하여 반복 재현성이 높은 뿐만 아니라 다형성 정 도가 높은 18개를 선정하였다. 이들 마커와 국내외에서 재배되고 있는 감귤 108품종을 검정하였을 때 마커당 평균 대립유전자 수는 9.28개로 나타났고, 5-14개까지 다양한 분포를 나타내었다. PIC 값은 분자표지에 따라 0.417-0.791 범위에 속하였으며 평균값은 0.606으로 나타났다. 감귤류 108품종에 대하여 계통도를 작성하였을 때 감귤류 식물의 분류학적 특성 및 품종 육성 의 계보도에 따라 13개의 그룹으로 크게 나누어졌다. 감귤류 식물 품종중 오렌지나 온주 밀감의 경우 대부분의 품종이 SSR 마 커의 유전자형에 의해 구분이 되지 않은 것으로 나타났다 .

본 연구에서 개발된 감귤속 식물의 품종별 SSR DNA 프로파일 데이터베이스는 감귤속 식물의 유전자원 특성평가와 육종 가의 지식재산권 보호의 수단으로 유용하게 활용될 수 있을 것이다.

키워드
감귤류
유전적 유연관계
초위성체
분자표지
유전자원
품종보호
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 34
  • No :1
  • Pages :142-153
  • Received Date : 2015-08-31
  • Revised Date : 2015-10-14
  • Accepted Date : 2016-01-25
  • DOI :https://doi.org/10.12972/kjhst.20160012
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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