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2017 Vol.35, Issue 3 Preview Page
Characterization of the Lsi1 Homologs in Cucurbita moschata and C. ficifolia for Breeding of Stock Cultivars Used for Bloomless Cucumber Production
Jaemin Jung1
,
Joonyup Kim3
,
Bingkui Jin1
,
Youngmi Choi1
,
Chang Oh Hong2
,
Hyun Ho Lee2
,
Jumsoon Kang1
,
Younghoon Park13*
1Department of Horticultural Bioscience, Pusan National University
2Department of Life Science and Environmental Biochemistry, Pusan National University
3Life and Industry Convergence Research Institute, Pusan National University
*교신저자. *Corresponding Author. 
30 June 2017. pp. 333-343
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Abstract

Bloomless cucumber fruits are commercially produced by grafting onto the pumpkin stocks (Cucurbita moschata ) to restricted silicon (SiO2) absorption. Inhibition of silicon absorption in bloomless stocks is conferred by a mutant allele of the CmLsi1 homologous to Lsi1 in rice. In this study, we characterized the Lsi1 homologs in pumpkin (C. moschata ) and its coldtolerant wild relative C. ficifolia (‘Heukjong’) in order to develop a DNA marker for selecting a bloomless trait and to establish the molecular basis for breeding bloomless stock cultivars of C. ficifolia . A Cleaved amplified polymorphic sequence (CAPS) marker (CM1-CAPS) was designed based on a non-sysnonymous single nucleotide polymorphism (SNP, C>T) of the CmLsi1 mutant-type allele, and its applicability for Marker-assisted selection (MAS) was confirmed by evaluating three bloom and five bloomless pumpkin stock cultivars. Quantitative RT-PCR of the CmLsi1 for these stock cultivers implied that expression level of the CmLsi1 gene does not appear to be associated with the bloom/bloomless trait and may differ depending on plant species and tissues. A full length cDNA of the Lsi1 homolog [named CfLsi1 (B+)] of ‘Heukjong’ (C. ficifolia ), was cloned and sequence comparison between CmLsi1 (B+) and CfLsi1 (B+) revealed that there exists total 24 SNPs, of which three were non-synonymous. Phylogenetic analysis of CfLsi1 (B+) and Lsi1 homologs further revealed that CfLsi1 (B+) is closesly related to Nodulin 26-like intrinsic proteins (NIPs) and most similar to CpNIP1 of C. pepo than C. moschata .

Keywords
cucurbitaceae
Lsi2
Low silicon rice 1
marker-assisted breeding
transporters
RACE PCR

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 35
  • No :3
  • Pages :333-343
  • DOI :https://doi.org/10.12972/kjhst.20170036
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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