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2014 Vol.32, Issue 6 Preview Page
Effect of Chlorella vulgaris CHK0008 Fertilization on Enhancement of Storage and Freshness in Organic Strawberry and Leaf Vegetables

Chlorella vulgaris CHK0008 시비가 유기농 딸기와 엽채소의 저장성과 신선도

Min-Jeong Kim1
,
Chang-Ki Shim1*
,
Yong-Ki Kim1
,
Jong-Ho Park1
,
Sung-Jun Hong1
,
Hyeong-Jin Ji1
,
Eun-Jung Han1
,
Jung-Chul Yoon1
김 민정1
,
심 창기1*
,
김 용기1
,
박 종호1
,
홍 성준1
,
지 형진1
,
한 은정1
,
윤 종철1
1Organic Agriculture Division, National Academy of Agricultural Science, Rural Development Administration
1농촌진흥청 국립농업과학원 유기농업과
*교신저자. *Corresponding Author. 
31 December 2014. pp. 872-878
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Abstract

This study aimed to enhance storage and freshness of strawberry fruits and foliage vegetables by spray treatment with Chlorella vulgaris as a bio-fertilizer. The tested strain, C. vulgaris CHK0008, was isolated from an organically cultivated rice paddy and identified as C. vulgaris by its morphology and 18S rDNA and 23S rDNA sequence homology. We successfully cultured C. vulgaris CHK0008 in BG11 modified medium (BG11MM) and adjusted 2.15 × 106 cell/mL C. vulgaris CHK0008 to one OD value by measuring the optical density at 680 nm using a UV-vis spectrophotometer. The soluble solid content of ‘Seolhyang’ and ‘Yukbo’ strawberry fruits treated by spray application with C. vulgaris CHK0008 was enhanced by 22.2% and 11.5% respectively, compared to untreated controls. Additionally, the decay rates of treated ‘Seolhyang’ and ‘Yukbo’ strawberry fruits decreased 63.8% and 74.4% respectively, compared to untreated control. Surface color changes and chlorosis of leaves in leaf vegetables such as lettuce, kale, red ornamental kale, white ornamental kale and beet were observed in samples treated with water spray for 10 days after cold storage. However, the decay rate of leafy vegetables treated with foliar application of 25% C. vulgaris CHK0008 liquid culture was significantly decreased compared to that of the untreated control during storage at 4°C.

Keywords
freshwater algae
fresh-cut vegetable
Seolhyang
spray and soil drenching application
Yukbo

이 연구는 생물비료로서 클로렐라(Chlorella vulgaris) 분무처리에 의한 유기농 딸기와 엽채류의 신선도와 저장성 향상을 목표로 하였다. 클로렐라 균주 CHK0008은 유기농 벼재배 논의 담수에서 분리하였으며 형태적 특징과 18S rDNA와 23S rDNA 염기서열 비교에 의해 C. vulgaris로 동정하였다. 실험에 사용한 C. vulgaris CHK0008 균주는 BG11 변형배지(BGMM)에서 잘 배양되었으며 UV/VIS 분광광도계로 680nm에서 흡광도를 측정하였을 때 1 OD의 C. vulgaris CHK0008는 2.15 × 106cell/mL 농도로 개산하였다. 클로렐라(C. vulgaris)의 엽면처리구와 무처리구를 비교하였을 때 ‘설향’과 ‘육보’ 딸기 품종의 당도가 각각 22.2%, 11.5% 향상되었다. 또한 엽면처리구의 ‘설향’과 ‘육보’ 딸기 품종의 부패율은 무처리구에 비해 각각 63.8%와 74.4% 감소하였다. 엽채류인 상추, 케일, 붉은 케일, 흰 케일, 비트의 엽색 변화 및 백화현상이 저온저장 10일 후 물만 분무 처리한 무처리에서 관찰되었다. 그러나 25% C. vulgaris 배양액을 엽면 처리한 엽채류를 4°C에서 저장하는 동안 부패율이 무처리에 비해 현저히 감소하였다.

키워드
담수조류
신선채소
설향
분무처리
토양관주
육보
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :한국원예학회
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 32
  • No :6
  • Pages :872-878
  • Received Date : 2014-06-16
  • Revised Date : 2014-07-09
  • Accepted Date : 2014-07-14
  • DOI :https://doi.org/10.7235/hort.2014.14107
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