FISH Mapping of Four Senna Species Revealing Genome Dynamics during Species Diversification of the Genus

Thi Minh Phuong Dinh; Byung Yong Kang; Hyun Hee Kim

doi:10.7235/HORT.20240015

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2024 Vol.42, Issue 2 Preview Page Next Page

Research Article

FISH Mapping of Four Senna Species Revealing Genome Dynamics during Species Diversification of the Genus

30 April 2024. pp. 180-187

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Abstract

Ploidy changes and chromosomal rearrangements significantly influence the chromosomal structure, which is a vital driver of speciation. The number and structure of the chromosomal composition of Senna species has been reported to vary in several cytogenetic studies. Here, we report chromosome number and structure variations among four Senna species through the fluorescence in situ hybridization (FISH) technique, utilizing ribosomal DNA (5S, 45S rDNAs) and telomeric repeat probes. Four distinct chromosome numbers, 2n = 26, 28, 48, and 56, were observed in S. obtusifolia, S. pallida, S. aphylla, and S. artemisioides, respectively, with basic chromosome numbers of x = 12, 13, and 14, likely corresponding to two diploid species (S. obtusifolia, S. pallida) and two tetraploid species (S. aphylla, S. artemisioides). The 5S rDNA signals varied in terms of both the number and position, and the 45S rDNA signals showed greater variation in the numbers, which ranged from two to eight. S. obtusifolia was distinct from the other three species in that its telomeric repeat positions varied considerably. While S. artemisioides, S. pallida, and S. aphylla exhibited telomeric signals primarily in the terminal regions, S. obtusifolia displayed these signals in the terminal, interstitial, and pericentromeric regions. In addition, interstitial telomeric repeat signals were observed in S. pallida. Our study demonstrates the first use of triple-color FISH to unravel molecular cytogenetic information in these Senna species, providing evidence of the extensive chromosomal rearrangement that occurred during the diversification of the genus Senna.

Keywords

chromosome evolution

diploidy

dysploidy

interstitial telomeric repeat (ITR)

repeat marker

tetraploid

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 42
No :2
Pages :180-187
Received Date : 2023-09-26
Revised Date : 2023-11-27
Accepted Date : 2023-12-10
DOI :https://doi.org/10.7235/HORT.20240015

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

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