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2022 Vol.40, Issue 3 Preview Page

Research Article

Responses of Antioxidant Enzyme Activity to Different Fertilizer and Soil Moisture Conditions in Relation to Cold Resistance in Zanthoxylum armatum
Jiangtao Fan1
,
Jingyan Wang1
,
Xianzhi Liu1
,
Changping Zhao1
,
Chaobing Zhou12
,
Tasheen Saba1
,
Jiaojiao Wu1
,
Wenkai Hui1
,
Wei Gong1*
1Sichuan Provincial Key Laboratory of Ecological Forestry Engineering, College of Forestry, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
2College of Biology and Agriculture, Zunyi Normal College, Zunyi, Guizhou 563006, China
*Corresponding Author
30 June 2022. pp. 261-272
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Abstract

Zanthoxylum armatum, known as green prickly ash, is a major condiment species planted on a large scale in southwest China. However, its limited cold tolerance has been a significant disadvantage for its cultivation in low-temperature regions. To better understand the antioxidant enzyme activities related to cold resistance, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities as well as malondialdehyde (MDA) content were investigated under 16 fertilizer and soil moisture treatments to explore the cold resistance response mechanism in Z. armatum. The results showed that low temperatures could significantly affect antioxidant enzyme activities in the leaves of Z. armatum seedlings. The antioxidant enzyme activities of seedlings treated with soil moisture and fertilizer treatments were significantly higher than those without fertilizer. Furthermore, the comprehensive evaluation index (CEI) was positively correlated with SOD, POD, and CAT activities but negatively correlated with MDA content. Meanwhile, the treatment T12 (60% field water capacity + 300 kg·ha-1 nitrogen + 30 kg·ha-1 phosphoric anhydride) had the highest CEI value, whereas T1 (20% water capacity) had the lowest. Based on the regression equation for CEI improvement, the predicted optimal levels for field water capacity (FWC), nitrogen, phosphorus, and potassium were 59.6%–63.4% FWC (average 61.5%), 114.2–248.8 kg N/ha (average 181.5 kg N/ha), 40.1–108.5 kg P2O5/ha (average 74.3 kg P2O5/ha), and 200.2–254.4 kg K2O/ha (average 227.3 kg K2O/ha), respectively. The present study provides the optimal soil moisture and fertilizer concentration required to enhance protective enzyme activity and low-temperature tolerance, which is crucial for improving cold resistance in Z. armatum.

Keywords
green prickly ash
orthogonal design
response surface analysis
stress resistance
water and fertilizer management
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 40
  • No :3
  • Pages :261-272
  • Received Date : 2022-03-12
  • Revised Date : 2022-03-16
  • Accepted Date : 2022-05-18
  • DOI :https://doi.org/10.7235/HORT.20220025
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