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2020 Vol.38, Issue 5 Preview Page

Research Article

QTL Mapping and molecular markers of Powdery Mildew Resistance in Pumpkin (Cucurbita moschata)

애호박(C. moschata)에서 흰가루병 저항성에 대한 QTL 분석 및 연관 분자표지 개발

Beom-Seok Park1*
,
Siyoung Jang2
,
Yeisoo Yu3
,
Gyung Ja Choi4
,
Byoung-Cheorl Kang2
,
SangKi Seo1
박 범석1*
,
장 시영2
,
유 의수3
,
최 경자4
,
강 병철2
,
서 상기1
1Research Institute, Hongikbio Inc.
2Department of Plant Science, Seoul National University
3DNACARE Co., Ltd.
4Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology
1농업회사법인 홍익바이오㈜
2서울대학교 식물생산과학부
3주식회사 디엔에이케어
4한국화학연구원 친환경신물질연구센터
* Corresponding Author
31 October 2020. pp. 717-729
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Abstract

Cucurbita moschata, an economically and nutritionally important vegetable in Korea, is seriously damaged in fruit quality and productivity by powdery mildew fungus (Podosphaera xanthii). To understand genetic bases of the powdery mildew disease resistance, an F2 population was developed from a cross between TG201 (C. moschata, susceptible, female parent) and TG10 (resistant, male parent) heirloom from Hongikbio Inc. Randomly selected 204 F2 individuals were assayed against powdery mildew pathogen, and construction of genetic map and QTL analysis were conducted using SNPs derived from genotyping-by-sequencing (GBS) method. The single major QTL was located in 6.9 ‑ 7.3 Mb region of TG10 chromosome 3. Within the 400 kb region, we investigated DNA insertion and deletion (indel) variations between TG201 and TG10 genome, and successfully designed seven sets of indel PCR markers. The functional validity of the powdery mildew resistance markers was confirmed with F2 individuals, breeding lines, and commercial varieties. From genomic sequence comparison on the basis of the breeding history, we also could clarify that the powdery mildew resistance region was introduced from C. okeechobeensis subsp. martinezii to C. moschata. Moreover, whole genome assemblies of two heirloom parents and genome-wide SNP/indel information were produced and are expected to be utilized as a genome resource for study of genus Cucurbita. The results and information of this study will be useful for genetics researches and breeding practices of C. moschata.

Keywords
Genotyping-by-sequencing (GBS)
introgression
SNP (single nucleotide polymorphism)
whole genome re-sequencing

이 연구에서는 우리나라의 중요 박과 작물인 애호박(동양계 호박, Cucurbita moschata)의 재배와 생산에 큰 피해를 주는 흰가루병(Podosphaera xanthii)의 저항성 유전자좌를 연구하였다. 홍익바이오(주)의 애호박 육종 소재 중에서 흰가루병 감수성 계통(TG201, ♀)과 저항성 계통(TG10, ♂)을 선정하고, 교배를 통하여 F2 집단을 만들었다. 그 중에 204개체를 대상으로 흰가루병 검정, GBS(genotyping-by-sequencing) 분석, SNP 유전지도 작성, QTL 분석을 수행하였다. 흰가루병 저항성 유전자좌는 TG10의 염색체 3번의 6.9 ‑ 7.3Mb 부분에 매핑되었다. 그리고 양친 유전체의 이 400kb 영역 내의 DNA 삽입/결실(indel)을 조사하고 7세트의 PCR 분자표지를 제작하였으며 F2 개체, 육종 소재 및 시판 품종을 대상으로 그 유효성을 검증하였다. 또한 실험에 사용된 애호박 양친의 유전체 염기서열 정보를 생산하고 해독하여 단일염기다형성(SNP) 분석을 수행하였다. 흰가루병 저항성 애호박 품종 육성 가계에 근거한 비교유전체 분석을 통하여 TG10이 갖고 있는 흰가루병 저항성 유전자좌 영역은 C. okeechobeensis subsp. martinezii로부터 이입된 염색체 토막의 일부임을 밝혔다. 본 연구에서 생산된 애호박 유전체 정보와 SNP 변이 데이터, 흰가루병 저항성 유전자좌 정보와 indel PCR 분자표지는 박과 식물의 연구와 개발에 유용하게 이용될 것으로 기대된다.

키워드
Genotyping-by-sequencing(GBS)
유전자 이입
단일염기다형성(SNP)
유전체 재염기서열
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :5
  • Pages :717-729
  • Received Date : 2020-05-27
  • Revised Date : 2020-06-22
  • Accepted Date : 2020-06-27
  • DOI :https://doi.org/10.7235/HORT.20200065
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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