All Issue

2021 Vol.39, Issue 1 Preview Page

Research Article

Phenotypic and Genetic Characterization of Three Different Types of Dangyooza (Citrus grandis), Korean Landrace Citrus
Kyung Uk Yi1
,
Kook Lhim Zhin2
,
Eun Ui Oh2
,
Sang Suk Kim3
,
Ho Bang Kim1
,
Kwan Jeong Song24†
1Life Sciences Research Institute, Biomedic Co., Ltd., Bucheon 14548, Korea,
2Faculty of Bioscience and Industry, SARI, Jeju National University, Jeju 63243, Korea,
3Citrus Research Institute, National Institute of Horticultural & Herbal Sciences, RDA, Jeju 63607, Korea
4Research Institute for Subtropical Agriculture & Biotechnology, Jeju National University, Jeju 63243, Korea
*Kwansong@jejunu.ac.kr
28 February 2021. pp. 96-105
PDF XML Citation
Abstract

Dangyooza (DY), a Korean landrace citrus similar to pomelo (Citrus grandis L. Osbeck), has been consumed as a fruit and used in folk remedies to treat colds for centuries, and it is still used as a garden tree. DY has two natural variants, the buk-daengyooza (BDY) and the seol-daengyooza (SDY) based on distinguishable phenotypic fruit characteristics. However, there is little scientific analysis available not only on the morphological and chemical properties but also on the genetic basis of these natural variants. To gain a better understanding of DY, several morphological, phytochemical, chromosomal, and genetic traits were analyzed in DY and its natural variants, BDY and SDY. Morphological characteristics such as leaf and fruit shape, fruit hardness, and peel thickness were used to discriminate SDY from DY and BDY. Notably, SDY produced smaller fruit with thinner peels than those of DY and BDY. The major flavanones occurring in citrus were also markedly higher in SDY than those of DY and BDY. However, chromosomal configuration and genetic diversity analysis using random amplified polymorphic DNA, simple sequence repeat, and plastid trnL/F barcoding markers were unable to clearly discriminate the phylogenetic relationships among the DY types. This suggests that SDY might have arisen from somatic mutation, perhaps as a nucellar seedling.

Keywords
genetic resources
karyotype
morphology
plastid DNA
RAPD
References
1
Abkenar AA, Isshiki S (2003) Molecular characterization and genetic diversity among Japanese acid citrus (Citrus spp.) based on RAPD markers. J Hortic Sci Biotechnol 78:108-112. doi:10.1080/14620316.2003.11511574 10.1080/14620316.2003.11511574
2
Abkenar AA, Isshiki S, Tashiro Y (2004) Phylogenic relationships in the "True Citrus Fruit Trees" revealed by PCR- RFLP analysis of cpDNA. Sci Hortic 102:233-242. doi:10.1016/j.scienta.2004.01.003 10.1016/j.scienta.2004.01.003
3
Ahmed S, Rattanpal HS, Singh G (2018) Diversity assessment of grapefruit (Citrus × paradisi) and tangelo (Citrus × tangelo) under Indian conditions using physic-chemical parameters and SSR markers. Appl Ecol Environ Res 16:5343-5358. doi:10.15666/aeer/1605_53435358 10.15666/aeer/1605_53435358
4
Baig MNR, Grewal S, Dhillon S (2009) Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers. Turk J Agric For 33:375-384
5
Barkley NA, Roose ML, Krueger RR, Federici CT (2006) Assessing genetic diversity and population structure in a citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theor Appl Genet 112:1519-1531. doi:10.1007/s00122-006-0255-9 10.1007/s00122-006-0255-916699791
6
Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultured Citrus and its close relatives. Syst Bot 1:105-136. doi:10.2307/2418763 10.2307/2418763
7
Befu M, Kitajima A, Hasegawa K (2001) Chromosome composition of some Citrus species and cultivars based on the chromomycin A3　(CMA) banding patterns. J Japan Soc Hortic Sci 70:83-88. doi:10.2503/jjshs.70.83 10.2503/jjshs.70.83
8
Befu M, Kitajima A, Yang X, Hasegawa K (2000) Classification of 'Tosa-Buntan' pummelo (Citrus grandis [L.] Osb.), 'Wash- ington' navel orange (C. sinensis [L.] Osb.), and triforiate orange (Poncirus trifoliata [L.] Raf.) chromosomes using young leaves. J Japan Soc Hortic Sci 69:22-28. doi:10.2503/jjshs.69.22 10.2503/jjshs.69.22
9
Benavente-Garcia O, Castillo J (2008) Update on uses and properties of Citrus flavonoids: new findings in anticancer, cardiovascular, and anti-inflammatory activity. J Agric Food Chem 56:6186-6205. doi:10.1021/jf8006568 10.1021/jf800656818593176
10
Biswas MK, Chai L, Amar MH, Zhang X, Deng XX (2011) Comparative analysis of genetic diversity in Citrus germplasm collection using AFLP, SSAP, SAMPL and SSR markers. Sci Hortic 129:798-803. doi:10.1016/j.scienta.2011.06.015 10.1016/j.scienta.2011.06.015
11
Carvalho R, dos Santos Soares Filho W, Brasileiro-Vidal AC, Guerra M (2005) The relationships among lemons, limes and citron: a chromosomal comparison. Cytogenet Genome Res 109:276-282. doi:10.1159/000082410 10.1159/00008241015753587
12
Chen CW, Huang YM, Kuo LY, Nguyen QD, Luu HT, Callado JR, Ferrar DR, Chiou WL (2013) trnL-F is a powerful marker for DNA identification of field vittarioid gametophytes (Pteridaceae). Ann Bot 111:663-673. doi:10.1093/aob/mct004 10.1093/aob/mct00423380240PMC3605945
13
Coletta-Filho HD, Machado MA, Targon MLPN, Pompeu Jr J (1998) Analysis of the genetic diversity among mandarins (Citrus spp.) using RAPD markers. Euphytica 102:133-139. doi:10.1023/A:1018300900275 10.1023/A:1018300900275
14
Dutt M, Vasconcellos M, Song KJ, Gmitter FG, Grosser JW (2010) In vitro production of autotetraploid Ponkan mandarin (Citrus reticulata Blanco) using cell suspension cultures. Euphytica 173:235-242. doi:10.1007/s10681-009-0098-y 10.1007/s10681-009-0098-y
15
El-Mouei R, Choumane W, Dway F (2011) Molecular characterization and genetic diversity in genus Citrus from Syria. Int J Agric Biol 13:351-356
16
Federici CT, Fang DQ, Scora RW, Roose ML (1998) Phylogenic relationships within the genus Citrus (Rutaceae) and related genera as revealed by RFLP and RAPD analysis. Theor Appl Genet 96:812-822 . doi:10.1007/s001220050807 10.1007/s001220050807
17
Food and agriculture organization of the United Nations (FAO) (2018) AOSTAT. Food and agriculture organization of the United Nations. Rome, Italy
18
Gattuso G, Barreca D, Gargiull C, Leuzzi U, Caristi C (2007) Flavonoid composition of citrus juices. Molecules 12:1641-1673. doi:10.3390/12081641 10.3390/1208164117960080PMC6149096
19
Guerra M (1993) Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining. Heredity 71:234-241. doi:10.1038/hdy.1993.131 10.1038/hdy.1993.131
20
Han SS, You IJ (1988) Studies on antimicrobial activities and safty of natural naringin in Korea. Korean J Mycol 16:1-8
21
Hertog MGL, Hollman PCH, Katan MB (1992) Content of potentially anticarcinogenic flavonoids of 28 vegetables and 9 fruits commonly consumed in the Netherlands. J Agric Food Chem 4:2379-2383. doi:10.1021/jf00024a011 10.1021/jf00024a011
22
Jena SN, Kumar S, Nair NK (2009) Molecular phylogeny in Indian Citrus L. (Rutaceae) inferred through PCR-RFLP and trnL-trnF sequence data of chloroplast DNA. Sci Hortic 119:403-416. doi:10.1016/j.scienta.2008.08.030 10.1016/j.scienta.2008.08.030
23
Jeong WS, Park SW, Chung SK (1997) The antioxidative activity of Korean Citrus unshiu peels. Food Biotechnol 6:292-296
24
Jung YH, Kwon HM, Kang SH, Kang JH, Kim SC (2005) Investigation of the phylogenetic relationships within the genus Citrus (Rutaceae) and related species in Korea using plastid trnL-trnF sequences. Sci Hortic 104:179-188. doi:10.1016/j.scienta.2004.08.008 10.1016/j.scienta.2004.08.008
25
Kang SH, Lee YJ, Lee CH, Kim SJ, Lee DH, LEE YK, Park DB (2005) Physiological activities of peel of Jeju-indigenous Citrus sunki Hort. Tanaka. Korean J Food Sci Technol 37:983-988
26
Kim HB, Jeon JH, Han AR, Lee Y, Jun SS, Kim TH, Cho GH, Park PB (2012) Genetic evaluation of domestic walnut cultivars trading on Korean tree markets using microsatellite markers. Afr J Biotechnol 11:7366-7374
27
Kim HY (1988) Distribution, taxonomy, horticultural characters of the local Citrus spp. in Cheju, and the genetic markers among them. PhD. Diss. Cheonnam Nat'l Univ, Korea
28
Kim YC, Koh KS, Koh JS (2001) Changes of flavonoids in the peel of Jeju native Citrus fruits during maturation. Food Sci Biotechnol 10:483-487
29
Li X, Xie R, Lu Z, Zhou Z (2010) The origin of cultivated citrus as inferred from internal transcribed spacer and chloroplast DNA sequence and amplified fragment length polymorphism fingerprints. J Am Soc Hortic Sci 135:341-350. doi:10.21273/JASHS.135.4.341 10.21273/JASHS.135.4.341
30
Lim HK, Yoo ES, Moon JY, Jeon YJ, Cho SK (2006) Antioxidant activity of extracts from Dangyuja (Citrus grandis Osbeck) fruits produced in Jeju Island. Food Sci Biotechnol 15:312-316
31
Liu KJ, Muse SV (2005) PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128-2129. doi:10.1093/bioinformatics/bti282 10.1093/bioinformatics/bti28215705655
32
Lu ZH, Zhou ZG, Xie RJ (2011) Molecular phylogeny of the "True citrus fruit trees" group (Aurantioideae, Rutaceae) as inferred from chloroplast DNA sequence. Agr Sci China 10:49-57. doi:10.1016/S1671-2927(11)60306-4 10.1016/S1671-2927(11)60306-4
33
Machado MA, Coletta Filho HD, Targon MLPN, Pompeu Jr J (1996) Genetic relationship of Mediterranean mandarins (Citrus deliciosa Tenore) using RAPD markers. Euphytica 92:321-326. doi:10.1007/BF00037115 10.1007/BF00037115
34
Miranda M, Ikeda F, Endo T, Morigushi T, Omura M (1997) Comparative analysis on the distribution of heterochromatin in Citrus, Poncitrus and Fortunella chromosomes. Chromosome Res 5:86-92. doi:10.1023/A:1018409922843 10.1023/A:10184099228439146911
35
Mokbel MS, Hashinaga F (2006) Evaluation of the antioxidant activity of extracts from buntan (Citrus grandis Osbeck) fruit tissues. Food Chem 94:529-534. doi:10.1016/j.foodchem.2004.11.042 10.1016/j.foodchem.2004.11.042
36
Moon DK, Kim KS, Kwon HM, Kim CM (2007) Citrus (Citrus spp., Rutaceae). In JM Lee, GW Choi, J Janick, eds, Horticulture in Korea. Korean Soc Hortic Sci Press, Suwon, pp 224-234
37
Nematollahi AK, Golein B, Vahdati K (2013) Analysis of the genetic diversity in citrus (Citrus spp.) species using SSR markers. J Plant Physiol Breeding 3:41-49
38
Nicolosi E, Deng ZN, Gentile A, La Malfa S, Continella G, Tribulato E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theor Appl Genet 100:1155-1166. doi:10.1007/s001220051419 10.1007/s001220051419
39
Nogata Y, Sakamoto K, Shiratsuchi H, Ishii T, Yano M, Ohta H (2006) Flavonoid composition of fruit tissues of citrus species. Biosci Biotechnol Biochem 70:178-192. doi:10.1271/bbb.70.178 10.1271/bbb.70.17816428836
40
Rahman MM, Nito N (1994) Phylogenetic relationships among the "True citrus fruit trees" by glutamate oxaloacetate transaminase isozymes analysis. J Jpn Soc Hortic Sci 62:755-760. doi:10.2503/jjshs.62.755 10.2503/jjshs.62.755
41
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233-234 10.1038/7270810657137
42
Scora RW (1988) Biochemistry, taxonomy and evolution of modern cultivated Citrus. Proc Int Soc Citricult VI Congr Vol 1, Margraf Publishers, Weikersheim, Germany, pp 277-289
43
Shrestha RL, Dhakal DD, Gautum DM, Paudyal KP, Shrestha S (2012) Genetic diversity assessment of acid lime (Citrus aurantifolia Swingle) landraces in Nepal, using SSR markers. Am J Plant Sci 3:1674-1681. doi:10.4236/ajps.2012.312204 10.4236/ajps.2012.312204
44
Tatum JH, Berry RE, Hearn CJ (1974) Characterization of citrus cultivars and separation of nucellar and zygotic seedlings by thin-layer chromatography. Proc Florida State Hortic Soc 87:75-81
45
Woo J, Park YC, Lee JW, Yun SH, Kim M, Park S, Lee Y, Song KJ, Kim HB (2019) Evaluation of polyembryony for genetic resources and efficacy of simple sequence repeat markers for the identification of nucellar and zygotic embryo-derived individuals in citrus. Appl Biol Chem 62:30. doi:10.1186/s13765-019-0437-1 10.1186/s13765-019-0437-1
46
Yamamoto M, Tominaga S (2003) High chromosomal variability of mandarins (Citrus spp.) revealed by CMA banding. Euphytica 129:267-274. doi:10.1023/A:1022284908424 10.1023/A:1022284908424
47
Yang YT, Kim HB, Lee SH, Park YC (2019) Composition characteristics of flavonoids in citrus juice. Hortic Sci Technol 37:10-19
48
Yi KU, Kim HB, Song KJ (2018a) Karyotype diversity of Korean landrace mandarins by CMA banding pattern and rDNA loci. Sci Hortic 228:26-32. doi:10.1016/j.scienta.2019.10.001 10.1016/j.scienta.2017.10.001
49
Yi KU, Kim HB, Song KJ (2018b) Cytogenetic phenogram with high resolution chromosome configurations of some Korean landrace citrus by CMA banding and rDNA loci. Sci Hortic 240:417-424. doi:10.1016/j.scienta.2018.06.058 10.1016/j.scienta.2018.06.058
50
Yildiz E, Kaplankiran M, Demirkeser TH, Uzun A, Toplu C (2013) Identification of zygotic and nucellar individuals produced from several citrus crosses using SSRs markers. Not Bot Horti Agrobo 41:478-484. doi:10.15835/nbha4129037 10.15835/nbha4129037
51
Yingzhi L, Yunjiang C, Nengguo T, Xiuxin D (2007) Phylogenetic analysis of mandarin landraces, Wild mandarins, and related species in China using nuclear LEAFY second intron and plastid trnL-trnF sequence. J Jpn Soc Hortic Sci 132:796-806. doi:10.21273/JASHS.132.6.796 10.21273/JASHS.132.6.796
52
Yoshimizu N, Otani Y, Saikawa Y, Kubota T, Yoshida M, Furukawa T, Kumai K, Kameyama K, Fujii M, et al. (2004) Anti-tumor effects of nobiletin, a citrus flavonoid, on gastric cancer include: antiproliferative effects, induction of apoptosis and cell cycle deregulation. Aliment Pharmacol Ther 1:95-101. doi:10.1111/j.1365-2036.2004.02082.x 10.1111/j.1365-2036.2004.02082.x15298613
53
Yun SH (2001) Classification of genus Citrus and its related genera using RAPD. Master Diss. Jeju Nat'l Univ, Jeju, Korea
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :1
  • Pages :96-105
  • Received Date : 2020-08-17
  • Revised Date : 2020-09-03
  • Accepted Date : 2020-09-14
  • DOI :https://doi.org/10.7235/HORT.20210009
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
Online Submission
  • scopus
  • NRF
  • KOFST
  • crossref crossmark
  • crossref cited-by
  • crosscheck
  • orcid
  • open access
  • ccl
Journal Informaiton Journal Informaiton - close