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2021 Vol.39, Issue 1 Preview Page

Research Article

Effects of Root Pruning on Adventitious Root Formation, Enzyme Activities, and Hormone Levels in Paeonia suffruticosa ‘Fengdanbai’ Seedlings
Zheng Wang1†
,
Gege Su1†
,
Songlin He12†
,
Liyun Shi1†
,
Dan He1
,
Wenqian Shang1
,
Dajuan Yang1
1College of Forestry, Henan Agricultural University, Zhengzhou 450002, China
2School of Horticulture Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China
*hsl213@yeah.net; sisyrin@126.com
†These authors contributed equally to this work.
28 February 2021. pp. 10-22
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Abstract

Root pruning during early root development can optimize the root structure of woody plants. To determine the effects of root pruning on tree peony (Paeonia suffruticosa), seedlings of the tree peony cultivar ‘Fengdanbai’ were subjected to varying degrees or different styles of root pruning. Various indexes were analyzed, including the anatomical structure of the root, root vitality, the activity levels of antioxidant enzymes, and the contents of endogenous hormones and their ratios. Comprehensive morphological observation showed that pruning freshly germinated roots that are 3 cm long by 1/3 (i.e., removing 1 cm) yielded the greatest rooting rate (90%), lateral root initiation (2.34 per seedling), and subsequent growth. Analysis of physiological and biochemical indicators during the critical rooting period (3-5 days after root pruning) showed that the levels of superoxide dismutase and abscisic acid (ABA) were decreased, while the oxidase activities of peroxidase, polyphenol oxidase, and indole acetate oxidase and the contents of the endogenous hormones indole acetic acid (IAA), gibberellin (GA3), and zeatin (ZT) were increased. IAA plays a key role in the process of adventitious root formation and the ratios of IAA/GA3, IAA/ABA, and IAA/ZT reflect endogenous hormone homeostasis during adventitious root formation. IAA/ABA and IAA/ZT showed higher values in the pruned roots compared to the control roots, which favor adventitious root induction and elongation. These results provide a basis for the study of adventitious root formation in tree peony.

Keywords
adventitious root
morphological index
paraffin sections
physiological indices
tree peony
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 39
  • No :1
  • Pages :10-22
  • Received Date : 2019-04-29
  • Revised Date : 2020-09-09
  • Accepted Date : 2020-09-24
  • DOI :https://doi.org/10.7235/HORT.20210002
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