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2019 Vol.37, Issue 6 Preview Page

Research Article

Development of Male-Sterile Elite Lines using Marker-Assisted Backcrossing (MABC) in Tomato

Marker-Assisted Backcrossing (MABC)을 이용한 토마토 웅성불임 엘리트 계통 육성

Dong Hyun Kim1†
,
Yu Jin Jung14†
,
Jong Hee Kim1
,
Hee Kyoung Kim1
,
Ki Hong Nam1
,
Hyo Ju Lee1
,
Myong-Kwon Kim2
,
Ill-Sup Nou3
,
Kwon Kyoo Kang14*
김 동현1†
,
정 유진14†
,
김 종희1
,
김 희경1
,
남 기홍1
,
이 효주1
,
김 명권2
,
노 일섭3
,
강 권규14*
1Department of Horticultural Life Science, Hankyong National University
2Tomato Research Center
3Department of Horticulture, Sunchon National University
3Institute of Genetic Engineering, Hankyong National University
1한경대학교 원예생명과학과
2(주)토마토연구소
3순천대학교 원예학과
3한경대학교 유전공학연구소
* Corresponding Author
† These authors contributed equally to this work.
31 December 2019. pp. 744-755
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Abstract

Recurrent backcrossing is a traditional breeding method that is commonly employed to transfer alleles at one or more loci from a donor to an elite variety. In order to develop male-sterile elite tomato lines, marker-assisted foreground and background selections were performed during backcross breeding. Compared to conventional backcrossing, marker-assisted backcrossing (MABC) is extremely useful for recovery of a recurrent parent’s genetic background. For foreground selection, the tomato mutant anthocyanin absent (aa) presents a green hypocotyl during the seedling stage. Lines carrying the Aa genotype were selected from BC1F1 and BC2F1 populations using indel markers derived from the unique aa mutation of T2-517. This trait has been utilized in marker-assisted selection of male sterile 10 (ms10) at the seedling stage because their corresponding loci are closely linked on chromosome 2. For background selection, a total of 48 single nucleotide polymorphism (SNP) markers obtained from resequencing data between the MR10-3211 and T2-517 lines evenly distributed in the tomato genome were finally selected. BC1F1 and BC2F1 plants carrying the heterozygous (Aa) genotype were subjected to background selection using a set of 48 SNP markers. Multiple genotype analysis was done using a high-throughput genotyping system. As a result, one plant, 87.5% similar to the recurrent parent genome in the BC1F1 generation, and three plants with a 95.5% recovery rate of the recurrent parent genome in the BC2F1 generation, were selected. These selected plants were fixed in the BC2F2 and BC2F3 generations for the male sterile tomato elite line. We therefore demonstrate the utility of the MABC method for the recovery of recurrent parent genomes in tomato breeding strategies.

Keywords
background selection
foreground selection
marker-assisted selection (MAS)
recurrent backcrossing
single nucleotide polymorphism (SNP) marker

Recurrent backcrossing은 donor친으로부터 엘리트 품종에 하나 이상의 좌에서 대립유전자들을 도입하려고 할 때 일반적으로 사용되는 전통적인 육종 방법이다. 웅성불임 엘리트 토마토 계통을 육성하기 위해 마커 지원 foreground 및 background 선발은 여교잡 육종 과정에서 수행하였다. MABC(marker-assisted backcrossing)은 기존의 여교배와 비교할 때 반복친의 유전적 배경을 회복하는데 매우 유용하게 사용하고 있다. Foreground 선발을 위해서 육묘 단계에 녹색 배축을 보이는 안토시아닌 결손(aa) 토마토 변이체는 T2-517 계통 유래 InDel 마커를 사용하여 BC1F1 및 BC2F1 집단에서 선발하였다. 이들 특성은 웅성불임 ms1035 유전자와 2번 염색체상에 매우 밀접하게 연관되어 있으므로 MAS(marker-assisted selection)에 이용하였다. Background 선발을 위해서 MR10-3211과 T2-517 계통 간의 resequencing 데이터로부터 얻은 48개의 single nucleotide polymorphism(SNP) 마커는 토마토 게놈에 고르게 분포되도록 최종 선발하였다. 이형접합(Aa) 유전자형을 갖는 BC1F1 및 BC2F1 개체들은 48개의 SNP 마커 세트를 background 선발에 이용하였다. 다중 유전형 분석은 high-throughput genotyping system(EP1TM, Fluidigm®, USA)을 사용하여 수행되었다. 그 결과 BC1F1 세대에서 반복친 게놈과 87.5%가 유사한 1개의 식물체와 BC2F1 세대에서 반복친 게놈의 회복률이 95.5% 이상으로 보이는 3개의 식물체를 선발하였다. 선발된 개체들은 토마토 웅성불임 계통을 육성하기 BC2F2 및 BC2F3 세대로 고정하였다. 따라서 MABC 방법은 토마토 육종 프로그램에서 반복친 게놈을 회복시키기 위한 획기적인 방법으로 생각된다.

키워드
background selection
foreground selection
marker-assisted selection (MAS)
반복여교잡 단일염기다형성(SNP) 마커
References
1
Atanassova B (2007) Genic male sterility and its application in tomato (Lycopersicon esculentum Mill.) hybrid breeding and hybrid seed production. Acta Hortic 729:45-51. doi:10.17660/ActaHortic.2007.729.3
10.17660/ActaHortic.2007.729.3
2
Bhatt RP, Biswas VR, Kumar N (2001) Heterosis, combining ability and genetics for vitamin C, total soluble solids and yield in tomato (Lycopersicon esculentum) at 1700 maltitude. J Agric Sci 137:71-75. doi:10.1017/S0021859601008838
10.1017/S0021859601008838
3
Chase CD, Ribarits A, Heberle-Bors E (2010) Male sterility. p 437-457. In EC Pua, MR Davey, eds, Plant Developmental Biology: Biotechnological Perspectives. Springer, Heidelberg, Germany. doi:10.1007/978-3-642-02301-9_21
10.1007/978-3-642-02301-9_21
4
Cheema DS, Dhaliwal MS (2005) Hybrid tomato breeding. J New Seeds 6:1-14. doi:10.1300/J153v06n02_01
10.1300/J153v06n02_01
5
Chen G, Ye X, Zeng F, Wang J, Yuan L, Zhu S, Hou J, Cheng Y, Wang C (2019) Characterization and utilization of a cytoplasmic male sterility line of Wucai (Brassica campestris L.). Hortic Environ Biotechnol 60:373-382. doi:10.1007/s13580-019-00127-7
10.1007/s13580-019-00127-7
6
Collard BC, Mackill DJ (2008) Marker-assisted selection: An approach for precision plant breeding in the twenty-first century. Philos Trans R Soc B 363:557-572. doi:10.1098/rstb.2007.2170
10.1098/rstb.2007.217017715053PMC2610170
7
DePristo MA, Banks E, Poplin R, Garimella KV, Maguire JR, Hartl C, Philippakis AA, Angel del G, Rivas MA, et al. (2011) A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat Genet 43:491-498. doi:10.1038/ng.806
10.1038/ng.80621478889PMC3083463
8
Foolad MR (2007) Genome mapping and molecular breeding of tomato. Int J Plant Genom Artic 2007:64358. doi:10.1155/2007/64358
10.1155/2007/6435818364989PMC2267253
9
Gardner RG, Panthee DR (2010) Grape tomato breeding lines: NC 1 grape, NC 2 grape, and NC 3 grape. HortScience 45:1887-1888. doi:10.21273/HORTSCI.45.12.1887
10.21273/HORTSCI.45.12.1887
10
Goldberg RB, Beals TP, Sanders PM (1993) Anther development: Basic principles and practical applications. Plant Cell 5:1217-1229. doi:10.1105/tpc.5.10.1217
10.1105/tpc.5.10.12178281038PMC160355
11
Gorguet B, Schipper D, van Lammeren A, Visser RGF, van Heusden AW (2009) ps-2, the gene responsible for functional sterility in tomato, due to non-dehiscent anthers, is the result of a mutation in a novel polygalacturonase gene. Theor Appl Genet 118:1199- 1209. doi:10.1007/s00122-009-0974-9
10.1007/s00122-009-0974-919219598
12
Hanson MR (1991) Plant mitochondrial mutations and male sterility. Annu Rev Genet 25:461-486. doi:10.1146/annurev.ge.25.120191. 002333
10.1146/annurev.ge.25.120191.0023331812814
13
Herzog E, Frisch M (2011) Selection strategies for marker-assisted backcrossing with high-throughput marker systems. Theor Appl Genet 123:251-260. doi:10.1007/s00122-011-1581-0
10.1007/s00122-011-1581-021476041
14
Jang SJ, Sato M1, Sato K, Jitsuyama Y, Fujino K, Mori H, Takahashi R, Benitez ER, Liu B, et al. (2015) A single-nucleotide polymorphism in an endo-1,4-β-glucanase gene controls seed coat permeability in soybean. PLoS ONE 10:e0128527. doi:10.1371/journal.pone.01 28527
10.1371/journal.pone.012852726039079PMC4454576
15
Jeong HJ, Kang JH, Zhao MA, Kwon JK, Choi HS, Bae JH, Lee HA, Joung YH, Choi D, et al. (2014) Tomato male sterile 1035 is essential for pollen development and meiosis in anthers. J Exp Bot 65:6693-6709. doi:10.1093/jxb/eru389
10.1093/jxb/eru38925262227PMC4246194
16
Jung YJ, Nou IS, Cho YG, Kim MK, Kim HK, Kang KK (2016) Identification of an SNP variation of elite tomato (Solanum lycopersicum L.) lines using genome resequencing analysis. Hortic Environ Biotechnol 57:173-181. doi:10.1007/s13580-016-0132-7
10.1007/s13580-016-0132-7
17
Kim JE, Lee BW, Kim SM, Lee BM, Lee JH, Jo SH (2013) Genome-wide SNP database for marker-assisted background selection in tomato. Korean J Breed Sci 45:232-239. doi:10.9787/KJBS.2013.45.3.232
10.9787/KJBS.2013.45.3.232
18
Lee SY, Kim SY, Kim DY, Jeong HJ, Um IS, Rho IR (2018) Effects of backcrossing on quality of strawberry fruit. Hortic Environ Biotechnol 59:225-230. doi:10.1007/s13580-018-0023-1
10.1007/s13580-018-0023-1
19
Mutschler MA, Rick CM, Tanksley SD (1987) Linkage maps of the tomato (Lycopersicon esculentum). Rep Tomato Genet Coop 37:5-34.
20
Panthee DR, Gardner RG (2013) 'Mountain Vineyard' hybrid grape tomato and its parents: NC 4 grape and NC 5 grape tomato breeding lines. HortScience 48:1189-1191. doi:10.21273/HORTSCI.48.9.1189
10.21273/HORTSCI.48.9.1189
21
Randhawa HS, Mutti JS, Kidwell K, Morris CF, Chen X, Gill KS (2009) Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection. PLoS ONE 4:e5752. doi:10.1371/journal.pone.0005752
10.1371/journal.pone.000575219484121PMC2684585
22
Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNAsepacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81:8014-8019. doi:10.1073/pnas.81.24.8014
10.1073/pnas.81.24.80146096873PMC392284
23
Servin B, Hospital F (2002) Optimal positioning of markers to control genetic background in marker assisted backcrossing. J Hered 93:214-217. doi:10.1093/jhered/93.3.214
10.1093/jhered/93.3.21412195040
24
Solieman THI, El-Gabry MAH, Abido AI (2013) Heterosis, potence ratio and correlation of some important characters in tomato (Solanum lycopersicum L.). Sci Hortic 150:25-30. doi:10.1016/j.scienta.2012.10.024
10.1016/j.scienta.2012.10.024
25
Su N, Hu ML, Wu DX, Wu FQ, Fei GL, Lan Y, Chen XL, Shu XL, Zhang X, et al. (2012) Disruption of a rice pentatrico peptide repeat protein causes a seedling-specific albino phenotype and its utilization to enhance seed purity in hybrid rice production. Plant Physiol 159:227-238. doi:10.1104/pp.112.195081
10.1104/pp.112.19508122430843PMC3366715
26
Tanksley SD, Nelson JE (1996) Advanced backcross QTL analysis: A method for simultaneous discovery and transfer of valuable QTL from unadapted germplasm into elite breeding. Theor App Genet 92:191-203. doi:10.1007/BF00223376
10.1007/BF0022337624166168
27
The Tomato Genome Consortium (2012) The tomato genome sequence provides insights into fleshy fruit evolution. Nature 485:635- 641. doi:10.1038/nature11119
10.1038/nature1111922660326PMC3378239
28
Venkatesh J, Song K, Lee J-H, Kwon J-K, Kang B-C (2018) Development of Bi gene-based SNP markers for genotyping for bitter-free cucumber lines. Hortic Environ Biotechnol 59:231-238. doi:10.1007/s13580-018-0029-8
10.1007/s13580-018-0029-8
29
Vidal RO, Nascimento LC, Mondego JMC, Pereira GAG, Carazzolle MF (2012) Identification of SNPs in RNA-seq data of two cultivars of Glycine max (soybean) differing in drought resistance. Genet Mol Biol 35:331-334. doi:10.1104/pp.112.195081
10.1104/pp.112.19508122430843PMC3366715
30
Zhang D, Yang L (2014) Specification of tapetum and micro-sporocyte cells within the anther. Curr Opin Plant Biol 17:49-55. doi:10. 1016/j.pbi.2013.11.001
10.1016/j.pbi.2013.11.00124507494
31
Zhang L, Huang Z, Wang X, Gao J, Guo Y, Du Y, Hu H (2016) Fine mapping and molecular marker development of anthocyanin absent, a seedling morphological marker for the selection of male sterile 10 in tomato. Mol Breed 36:107. doi:10.1007/s11032-016-0531-6
10.1007/s11032-016-0531-6
Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :6
  • Pages :744-755
  • Received Date : 2019-06-10
  • Revised Date : 2019-08-22
  • Accepted Date : 2019-09-02
  • DOI :https://doi.org/10.7235/HORT.20190074
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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