Development and Application of Polymorphic SSR Markers in Luffa cylindrica

Junjie Cui; Zhen Lv; Tianwen Yang; Jing Wang; Yu Hong; Yi Cao

doi:10.7235/HORT.20220049

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2022 Vol.40, Issue 5 Preview Page Next Page

Research Article

Development and Application of Polymorphic SSR Markers in Luffa cylindrica

31 October 2022. pp. 552-560

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Abstract

Two published genomes of Luffa cylindrica, ‘SG2019’ and ‘SO3’, were used to develop polymorphic SSR markers for L. cylindrica by searching and comparing the SSR motifs at the same site in the two genomes. Based on the SSR search conditions and primer design criteria, 2130 polymorphic SSR markers for L. cylindrica were developed. The main motif type was dinucleotide, accounting for 80.28% of the total; the main motif units were AT/AT and AAT/ATT, accounting for 87.80% of the total. Furthermore, 40 polymorphic SSR markers developed in this study were randomly selected and amplified in 24 Luffa samples. According to the results, the 40 polymorphic SSR markers showed an amplification rate of 100% and a polymorphism rate of 80%. Cluster analysis classified the 24 Luffa samples into two main groups, L. cylindrica and L. acutangula. Overall, the polymorphic SSR markers developed in this study display a high polymorphic rate and reliable utilization value.

Keywords

genetic diversity

genomic prediction

polymorphic information content

sponge gourd

SSR motif repeat

validation of polymorphism

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Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 40
No :5
Pages :552-560
Received Date : 2022-05-12
Revised Date : 2022-07-05
Accepted Date : 2022-07-18
DOI :https://doi.org/10.7235/HORT.20220049

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

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