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2019 Vol.37, Issue 5 Preview Page

Research Article

Growth, Antioxidant Concentrations and Activity in Sedum takesimense as Affected by Supplemental LED Irradiation with Light Quality

LED 광질에 따른 섬기린초의 생육과 항산화물질 함량 및 활성 분석

Sang Im Oh1
,
Ja Hee Lee1
,
Ae Kyung Lee1*
오 상임1
,
이 자희1
,
이 애경1*
1Department of Environmental Horticulture, Dankook University
1단국대학교 환경원예학과
* Corresponding Author
31 October 2019. pp. 589-597
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Abstract

This study was conducted to investigate the effect of different qualities of LED irradiation on the growth of and antioxidant concentrations and activity in Sedum takesimense. After blue LED treatment, leaf length and width increased, and there were more leaves per stem than under other treatments. The plants under RBW (red:blue:white = 1:1:1) LED treatment had significantly higher fresh and dry weights than those under other treatments. The concentrations of total flavonoids and total phenolics and DPPH (2,2 Diphenyl-1-picrylhydrazyl) radical scavenging activity of the plants increased after light treatment. The concentration of antioxidants (total flavonoids and total phenolics) and DPPH radical scavenging, ABTS [2, 2ʹ-Azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)] radical scavenging were higher after blue LED treatment than after any other treatment. The concentration of total flavonoids was highly correlated with that of total phenolics (r = 0.821, p ≤ 0.05) and with DPPH radical scavenging (r = 0.505, p ≤ 0.05), ABTS radical scavenging (r = 0.767, p ≤ 0.01). The concentration of total phenolics was also strongly correlated with DPPH radical scavenging (r = 0.868, p ≤ 0.01) and ABTS radical scavenging (r = 0.862, p ≤ 0.01) activities. It is suggested that blue LED treatment affects the synthesis of antioxidants during growth in chamber. We thus conclude that these antioxidant compounds are a major factor in determining antioxidant activity. Therefore, it was determined that Sedum takesimense belonging to Crassulaceae can be grown using blue LED in growth chambers.

Keywords
ABTS radical scavenging
blue LED
Crassulaceae
DPPH radical scavenging
flavonoid
phenolic

본 연구는 돌나물과 식물 중 섬기린초가 LED 광질 처리에 따라 생육과 항산화물질 함량 및 활성에 미치는 영향에 대하여 알아보고자 수행하였다. 섬기린초를 LED 광질에 따라 처리 한 후 생육 조사를 한 결과, blue LED 단색광 및 혼합광 처리 시 엽생장에 효과적이었으며, RBW LED 혼합광 처리 시 생체중과 건물중이 높은 것으로 조사되었다. 또한, LED 처리 시 총 플라보노이드 함량과 총 페놀 함량, DPPH radical 소거 활성은 처리 전보다 증가하는 경향을 보였다. 특히, blue LED 단색광 및 혼합광 처리가 다른 처리보다 총 플라보노이드와 총 페놀 함량이 높은 것으로 조사되었다. DPPH radical 및 ABTS radical 소거 활성을 통해 항산화 활성을 조사한 결과, 항산화물질 함량이 높았던 blue LED 처리가 활성이 높은 것으로 조사되었다. 이에 따라 blue LED 처리 후 섬기린초의 항산화물질 함량과 활성의 상관관계를 조사한 결과, 총 플라보노이드와 총 페놀은 서로 간의 상관관계가 높고(r = 0.821, p ≤ 0.05), DPPH radical 및 ABTS radical 소거 활성과도 상관관계를 가지는 것으로 조사되었다(r = 0.505, p ≤ 0.05; r = 0.767, p ≤ 0.01). 또한, 총 페놀과 DPPH radical 및 ABTS radical 소거 활성은 각각 r = 0.868, 0.862 (p ≤ 0.01)로 상관관계가 높은 것으로 나타났다. 따라서, blue LED 처리는 섬기린초의 엽생장에 효과적이며, 항산화물질 합성에 영향을 미치고 이로 인해 생성된 항산화물질은 항산화 활성의 주요 작용인자로 기여하는 것으로 판단된다. 또한, 위의 결과를 통해 챔버 내 섬기린초의 생육이 가능하며 blue LED 처리 시 생육과 기능성 물질 합성에 효과적인 것으로 판단된다.

키워드
ABTS radical 소거능
blue LED
돌나물과
DPPH radical 소거능
플라보노이드
페놀
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :5
  • Pages :589-597
  • Received Date : 2019-09-18
  • Revised Date : 2019-03-12
  • Accepted Date : 2019-05-16
  • DOI :https://doi.org/10.7235/HORT.20190059
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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