Establishment and Optimization of an in vitro Regeneration System in Shredded Pineapple

Jing Zhang; Xueli Fu; Yao Su; Tao Xie

doi:10.7235/HORT.20230021

All Issue

2023 Vol.41, Issue 2 Preview Page

Research Article

Establishment and Optimization of an in vitro Regeneration System in Shredded Pineapple

30 April 2023. pp. 224-235

PDF XML

Abstract

Shredded pineapple, a type of rare high-quality fresh pineapple, is convenient to eat and has high economic value worldwide. In this study, the crown buds and sucker buds of shredded pineapple were used to study the effects of different explants and the ratios of different hormones (i.e., 6-benzylaminopurine, 6-BA; naphthaleneacetic acid, NAA; and indolebutyric acid, IBA) on the induction, proliferation, differentiation, and rooting of pineapple, and the effects of seedling refining on the survival rate of transplants, to establish a regeneration system for shredded pineapple. The results showed that the most suitable explant was crown buds, the optimal medium for adventitious bud induction was MS+3.0 mg/L 6-BA+3.0 mg/L NAA, the optimal proliferation medium was MS+3.0 mg/L 6-BA+1.0 mg/L NAA, the optimal adventitious bud growth was the MS medium, and the optimal rooting medium was MS+2.0 mg/L IBA. The peak survival rate of regenerated plants was observed after seven days of seedling refining. This study successfully established and optimized a tissue culture and rapid propagation regeneration system for shredded pineapple, which provides technical support for the low-cost rapid production and genetic transformation of pineapples.

Keywords

adventitious bud

callus induction

fruits

hormones

tissue culture

References

Aghion D, Beauchesne G (1960) The use of the sterile tissue culture technique to obtain pineapple clones. Fruits Outre Mer 15:464-466

Anjanappa RB, Gruissem W (2021) Current progress and challenges in crop genetic transformation. J Plant Physiol 261:153411-153424. doi:10.1016/j.jplph.2021.153411 10.1016/j.jplph.2021.15341133872932

Baskaran P, Jayabalan N (2009) In vitro regeneration of Psoralea corylifolia L. through callus cultures. Plant Biotechnol 26:333-336. doi:10.5511/plantbiotechnology.26.333 10.5511/plantbiotechnology.26.333

Carlier JD, d'Eeckenbrugge GC, Leitão JM (2007) Pineapple. In Fruits and Nuts, Springer, Heidelberg, Berlin, Germany, pp 331-342. doi:10.1007/978-3-540-34533-6_18 10.1007/978-3-540-34533-6_18

Chen H (2018) Present situation and development trend of plant tissue culture technology. Biol Chem Eng 4:137-139 (in Chinese)

Elmongy MS, Zhou H, Cao Y, Liu B, Xia Y (2018) The effect of humic acid on endogenous hormone levels and antioxidant enzyme activity during in vitro rooting of evergreen azalea. Sci Hortic 227:234-243. doi:10.1016/j.scienta.2017.09.027 10.1016/j.scienta.2017.09.027

Fan ZT, Wei F, Qiao Z, Liang Y, Xie TG, Liu JH, Wei KH (2022) Research progress in the production of secondary metabolites by tissue culture of medicinal plants. Biol Resour 44:130-140 (in Chinese)

Harahap F, Diningrat DS, Poerwanto R, Nasution NEA, Hasibuan RFM (2019) In vitro callus induction on Sipahutar pineapple (Ananas comosus L.) from North Sumatra Indonesia. Pak J Biol Sci 22:518-526. doi:10.3923/pjbs.2019.518.526 10.3923/pjbs.2019.518.52631930830

Harahap F, Nusyirwan N, Hasanah A, Edi S, Rahayu S, Hasibuan R F M (2021) Pineapple rooting in vitro from Sipahutar, North Sumatra, Indonesia, with addition of sucrose and IBA. J Hunan Univ Nat Sci 48:7

He YH, Fang SQ, Ma J, Hu ZY, Lu M, Peng B (2010) Histocytology observation on the somatic embryogenesis in Ananas comosus callus. Acta Horticulturae Sinica 37:689-696 (in Chinese)

He YH, Luo J, Wu HT, Wang RX, Gao AP, Zhao CX, Yu XL, Ye ZX, Wang ZH, et al. (2007) Somatic embryogenesis from leaf base callus of Ananas comosus. J Fruit Sci 01:59-63 (in Chinese)

Huang DG, Chen YQ, Guo JH, Lin YY (1993) Study on tissue culture and rapid propagation of peeling-pineapple seedlings. Fujian Sci Technol Trop Crops Z1:1-10 (in Chinese)

Ikeuchi M, Ogawa Y, Iwase A, Sugimoto K (2016) Plant regeneration: cellular origins and molecular mechanisms. Development 143:1442-1451. doi:10.1242/dev.134668 10.1242/dev.13466827143753

Lin YF, Gao C, Zhang D, Liu Y, Xia YP, Ren ZM (2022) Regeneration of bulblet in vitro and preliminary study on genetic transformation system construction of Lycoris species. Mol Plant Breed 1-17 (in Chinese)

Luan A, Chen C, Xie T, He J, He Y (2020) Methylation analysis of CpG islands in pineapple SERK1 promoter. Genes (Basel) 11:425. doi:10.3390/genes11040425 10.3390/genes1104042532326553PMC7231283

Luo LX, Cheng P (2002) Study on tissue culture and macropropagation of peeling-pineapple. Chin Bull Bot 02:231-233 (in Chinese)

Min LM, Zhang JY, Wu WJ (2014) Tissue culture and rapid propagation of Ananas comosus 'Apple'. J Trop Biol 5:277-279 (in Chinese)

Mostafa HH, Wang H, Song J, Li X (2020) Effects of genotypes and explants on garlic callus production and endogenous hormones. Sci Rep 10:1-11. doi:10.1038/s41598-020-61564-4 10.1038/s41598-020-61564-432184427PMC7078195

Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473-497. doi:10.1111/j.1399-3054.1962.tb08052.x 10.1111/j.1399-3054.1962.tb08052.x

Nordström A, Tarkowski P, Tarkowska D, Norbaek R, Åstot C, Dolezal K, Sandberg G (2004) Auxin regulation of cytokinin biosynthesis in Arabidopsis thaliana: a factor of potential importance for auxin-cytokinin-regulated development. Proc Natl Acad Sci U S A 21:8039-8044. doi:10.1073/pnas.0402504101 10.1073/pnas.040250410115146070PMC419553

Oseni OM, Pande V, Nailwal TK (2018) A review on plant tissue culture, a technique for propagation and conservation of endangered plant species. Int J Curr Microbiol Appl Sci 7:3778-3786. doi:10.20546/ijcmas.2018.707.438 10.20546/ijcmas.2018.707.438

Pradhan K, Rout S, Tripathy B, Mishra UN, Sahoo G, Prusty AK, Dash L (2021) Role of Biotechnology in Vegetable Breeding. Turkish Online J Qual Inq 12:5092-5102

Priyanka G (2013) Plant tissue culture of Stevia rebaudiana (Bertoni): A review. J Pharmacognosy and Phytotherapy 2:26-33

Rahman KW, Amin MN, Azad MAK (2001) In vitro rapid clonal propagation of pineapple, Ananas comosus (L.) Merr. Plant Tiss Cult 11:47-53

Reinhardt DHR, Bartholomew DP, Souza FVD, Carvalho ACPPD, Pádua TRPD (2018) Advances in pineapple plant propagation. Rev Bras Frutic 40. doi:10.1590/0100-29452018302 10.1590/0100-29452018302

Sahebi M, Hanafi MM, Azizi P (2016) Application of silicon in plant tissue culture. In Vitro Cell Dev Biol Plant 52:226-232. doi:10.1007/s11627-016-9757-6 10.1007/s11627-016-9757-6

Sheng QQ (1991) Tissue culture and rapid propagation of Ananas comosus. J Plant Physiol 06:423-425 (in Chinese)

Šípošová K, Kollárová K, Lišková D, Vivodová Z (2019) The effects of IBA on the composition of maize root cell walls. J Plant Physiol 239:10-17. doi:10.1016/j.jplph.2019.04.004 10.1016/j.jplph.2019.04.00431177026

Wang X, Xu XL, Bian LL, Yao WJ, Lin SY (2021) Study on bud propagation and plant regeneration of Sasa glabra f. albastriata Muroi. Jiangsu Agri Sci 49:64-71 (in Chinese)

Yan HX, Guan SK, Zhou JY, Tao DY, La YF, Song Q (2021) Transplanting season of 17 species of Primulina. Chin J Trop Crops 42:1378-1386 (in Chinese)

Yang DY, Zhang LZ, Li BL (2011) Study on effective regeneration system of Ananas comosus sp. Anhui Agri Sci Bull (Second half monthly) 17:21-24 (in Chinese)

Yao N, Ma XR, Gao JZ, Qiao YR, Xu TT, Liang WY (2022) Establishment of tissue culture and plant regeneration system of Stellaria dichotoma. Mol Plant Breed 1-14 (in Chinese)

Zhang Y, Liu YQ, Su WQ (2010) Tissue culture and rapid propagation technology for pineapple variety Tainong 17. J South Agric 41:310-312 (in Chinese)

Zhang YF, Yang WX, Zhao WF (2017) Research Progress on in vitro propagation of pineapple. Mol Plant Breed 15:2472-2478 (in Chinese)

Zhang ZW (2011) Tissue culture and rapid propagation of pineapple (brief report). Jilin Agric 04:110 (in Chinese)

Zheng JX, Li HD, Gan YH (2005) Studies on tissue culture and rapid propagation with low cost of pineapple cultivar Mibao. J Fruit Sci 22:27-30 (in Chinese)

Zhou JK, Liu Y, Wu L, Zhao YL, Zhang W, Yang GY, Xu ZG (2021) Effects of plant growth regulators on the rapid propagation system of Broussonetia papyrifera L. Vent explants. Forests 12:874-879. doi:10.3390/f12070874 10.3390/f12070874

Information

Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
Publisher(Ko) :원예과학기술지
Journal Title :Horticultural Science and Technology
Journal Title(Ko) :원예과학기술지
Volume : 41
No :2
Pages :224-235
Received Date : 2022-07-07
Revised Date : 2023-01-19
Accepted Date : 2023-03-15
DOI :https://doi.org/10.7235/HORT.20230021

Horticultural Science and Technology ISSN:1226-8763(Print) 2465-8588(Online) 원예과학기술지

All Issue