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2019 Vol.37, Issue 4 Preview Page

Research Article

Identification and Control of Microbial Contaminants during in vitro Culturing of ‘Atlantic’ Potatos

‘대서’ 감자의 조직배양 동안 발생하는 미생물 오염원의 동정과 제어

Se-Hwi Gwon1
,
Jae-Yun Heo1*
,
Byung-Sup Kim1
권 세휘1
,
허 재윤1*
,
김 병섭1
1Department of Plant Science, Gangneung-Wonju National University
1강릉원주대학교 식물생명과학과
* Corresponding Author
31 August 2019. pp. 528-539
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Abstract

In order to gain preliminary information for developing a prevention system against microbial contamination during the micropropagation of the ‘Atlantic’ potato, we isolated bacteria and fungi that are involved in the process of micropropagation, and investigated the effects of antibiotic and fungicide treatments on the growth of fungi and bacteria. During the experiment, microbial contaminants were identified using their morphological characteristics and molecular analysis. Five bacteria and four fungi were identified. Determined contaminants were artificially isolated and subjected to different concentrations of antibiotics or fungicides to test their sensitivities against the contaminants. Although no single antibiotic treatment was effective in eliminating the identified bacteria, the application of some antibiotics reduced the severity of the problem. In contrast, growth of the fungi isolated from this research was significantly inhibited by the treatment of pyraclostrobin or fluazinam. In addition, the combined application of some antibiotics and fungicides, such as ‘vancomycin + pyraclostrobin’ and ‘penicillin G + fluazinam’, during micropropagation prevented the phytotoxicity of chemical agents in plants. These results indicate that the application of chemical agents can be useful for preventing microbial contamination during the micropropagation of the ‘Atlantic’ potato.

Keywords
asepsis
chemical agent
growth characteristics
plant tissue culture
Solanum tuberosum

본 연구에서는 ‘대서(Atlantic)’ 감자의 미세증식 과정 중 발생할 수 있는 오염에 대한 예방 체계를 만들기 위한 기초 자료를 얻기 위해서 미세증식 과정 중 발생할 수 있는 세균과 균류를 조사하고, 항생제와 살균제의 처리가 세균과 균류의 성장에 미치는 영향을 검정하였다. 실험과정 중 발생한 미생물 오염원들은 형태학적 특징과 분자 분석기법을 이용하여 동정하였으며, 그 결과 5종의 세균과 4종의 균류가 발생한 것으로 확인하였다. 동정된 오염원들은 인위적으로 분리되었으며, 공시된 항생제와 살균제에 대한 감수성을 검정을 위해 사용되었다. 비록 단일 항생제의 처리는 본 실험과정 중 동정된 세균의 제거에 있어서 크게 효과적이지 않았지만, 일부 항생제의 처리는 오염에 따른 문제의 심각성을 줄일 수는 있었다. 이와 대조적으로, 본 연구로부터 동정된 균류의 생장은 pyraclostrobin 또는 fluazinam의 단용 처리를 통해서 크게 억제되었다. 아울러, 미세증식 과정 중 ‘vancomycin과 pyraclostrobin’ 그리고 ‘penicillin G과 fluazinam’와 같은 항생제와 살균제의 혼용처리는 식물체에게 어떠한 독성작용도 일으키지 않았다. 이러한 결과는 화학약제의 적용이 ‘대서’ 감자의 미세증식 과정 중에 발생할 수 있는 미생물에 따른 오염을 예방하기 위한 효과적인 방법으로서 사용될 수 있음을 시사하는 것이다.

키워드
무균관리
화학약제
생육특성
식물조직배양
Solanum tuberosum
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 37
  • No :4
  • Pages :528-539
  • Received Date : 2019-02-19
  • Revised Date : 2019-05-11
  • Accepted Date : 2019-05-16
  • DOI :https://doi.org/10.7235/HORT.20190053
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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