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2020 Vol.38, Issue 2 Preview Page

Research Article

Effect of Tobacco Mosaic Virus (TMV) Infection on Expression and Purification of Therapeutic Vaccine Proteins in Transgenic Plants
Chunha Shin1†
,
Ilchan Song1†
,
Yeji Lee1
,
So-Hyeon Baek2
,
Heon Kim3
,
Kisung Ko1*
1Department of Medicine, College of Medicine, Chung-Ang University
2Department of Well-being Resources, Sunchon National University
3Department of Biology, Sunchon National University
* Corresponding Author
† These authors contributed equally to this work.
30 April 2020. pp. 282-290
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Abstract

Plant molecular biofarming has been increasingly studied in recent decades. Many kinds of recombinant immunotherapeutic proteins have been produced in transgenic tobacco plants. However, tobacco mosaic virus (TMV) can damage tobacco plants and cause pathogenic symptoms, which affects plant biomass production. Cigarette sap solution has been used to infect TMV during tobacco cultivation. In this study, we obtained TMV-infected transgenic plants expressing a prostate- specific antigen (PSA) fused to an IgG Fc with KDEL ER retention motif (PSA-IgG FcK). The typical TMV-associated symptoms appeared and increased on plant leaves 5 days after infection. mRNA expression levels and the presence of TMV were confirmed by RT-PCR and transmission electron microscopy analyses. The expression level and purity of the target protein were not significantly different between noninfected and infected transgenic plants. TMV was not found in the purified protein samples from infected plants. Our study showed that TMV pathogen-infected plant biomass can be harvested and processed to obtain therapeutic vaccine proteins.

Keywords
Agrobacterium-mediated transformation
fusion antigen
molecular biofarming
prostate cancer
prostate-specific antigen

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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :2
  • Pages :282-290
  • Received Date : 2019-11-12
  • Revised Date : 2019-12-20
  • Accepted Date : 2020-01-19
  • DOI :https://doi.org/10.7235/HORT.20200027
Journal Informaiton Horticultural Science and Technology Horticultural Science and Technology
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