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Research Article

Changes in Fruit Characteristics and Expression of Ripening-Related Genes in ‘Madoka’ Peaches Treated with 1-Methylcyclopropene and Aminoethoxyvinylglycine

1-MCP 및 AVG 수체처리에 따른 ‘마도카’ 복숭아 과실의 저장 중 특성 변화 및 성숙 관련 유전자 발현

Dan Bi Lee1
,
Guk Jin Lee1
,
Seung Heui Kim2
,
n Kyu Kang3
,
Seong Jin Choi4
,
Hae Keun Yun1*
이 단비1
,
이 국진1
,
김 승희2
,
강 인규3
,
최 성진4
,
윤 해근1*
1Department of Horticulture and Life Science, Yeungnam University
2Department of Fruits, Korea National College of Agriculture and Fisheries
3Department of Horticultural Science, Kyungpook National University
4Department of Biotechnology, Daegu Catholic University
1영남대학교 원예생명과학과
2한국농수산대학 과수학과
3경북대학교 원예과학과
4대구가톨릭대학교 생명공학과
* Corresponding Author
28 February 2020. pp. 32-43
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Abstract

Changes of the characteristics were investigated during storage of peach fruits following foliar application of 1-methylcyclopropene (1-MCP, 120 mg∙L-1 and 240 mg∙L-1), aminoetoxyvinylglcine (AVG, 75 mg∙L-1), and a combination of 1-MCP (240 mg∙L-1) and AVG (75 mg∙L-1) onto peach trees 3 weeks before harvesting. There were no significant differences in fruit characteristics including total soluble solids, acid content, and Hunter’s value a between control and 1-MCP- or AVG-treated fruits of ‘Madoka’ peaches during ambient (25°C) and cold storage (12°C). However, we observed a delayed decrease in firmness, as well as inhibition of ethylene production and respiration during storage of peach fruits after foliar application of 1-MCP, AVG, and AVG combined with 1-MCP in 2017 and in 2018 compared to untreated peach fruits. Quantitative real-time PCR using RNA isolated from peach fruits revealed significantly inhibited expression of sugar accumulation-related genes (polygalacturonase, PpPG and PpPGL; and lipoxygenase, PpLOX), cell wall softening-related gene (pectin methylesterase, PpPME), ethylene biosynthesis-promoting genes (ACC synthase, PpACS1; and ACC oxidase, PpACO), and genes responsive to ethylene receptors (ethylene insensitive protein, PpEIN; and ethylene response sensor 1, PpERS1) in fruits treated with 1-MCP or AVG after harvest. This suggests that foliar application with 1-MCP or AVG can extend the shelf-life of peaches through inhibition of ethylene production and respiration. These results are informative for the development of technology necessary to extend shelf-life, which may ultimately lead to increased consumption and export to foreign countries of peaches produced in Korea.

Keywords
ethylene
quantitative real-time PCR
respiration
shelf-life
softening

1-Methylcyclopropene(1-MCP, 120mg·L-1, 240mg·L-1)와 aminoetoxyvinylglycine(AVG, 75mg·L-1) 및 AVG(75mg·L-1) + 1-MCP(240mg·L-1)를 수확 3주 전에 처리하여 중생종 ‘마도카’ 복숭아의 저장 중 특성 변화를 조사하였다. 과실 수확 후 상온 저장과 저온 저장 중 가용성 고형물 함량, 산 함량, 과피색 변화 등의 과실특성은 처리와 무처리 간의 차이가 나타나지 않았다. 수확 직후의 과실에서도 1-MCP와 AVG를 처리한 과실에서는 경도가 높았으며, 저장 기간이 경과하면서 1-MCP와 AVG를 처리한 과실에서 연화가 억제되었다. 에틸렌 발생은 수확 직후에도 1-MCP와 AVG를 처리한 과실에서 무처리구에 비해 현저하게 낮았으며, 상온 저장과 저온저장 조건에서 모두 저장 일수가 경과함에 따라 무처리구에서 가장 높았으며 1-MCP와 AVG를 처리한 모든 과실에서 낮게 나타났다. 1-MCP와 AVG 를 처리한 후 저장 중인 과실에서 저장 일 수가 경과함에 따라 상온 저장과 저온 저장 중인 과실에서 모든 처리구에서 호흡률이 감소하였다. 1-MCP와 AVG 를 처리한 과실에서 RNA를 분리하여 real-time-PCR을 수행한 결과 당대사에 관여하는 유전자(PpPG, PpPGL, PpLOX), 세포벽 연화와 관련된 유전자(PpPME)는 물론 에틸렌 합성(PpACS1, PpACO) 과 수용체의 반응에 관여하는 유전자(PpEIN, PpERS1)의 발현이 억제되었다. 1-MCP와 AVG의 복숭아 수체 처리에 의해 과실의 에틸렌 합성과 호흡의 감소를 유도함으로써 저장 기간의 연장을 가져올 수 있을 것으로 여겨진다. 이러한 결과는 복숭아의 보구력을 향상시키고 저장 기간을 연장하기 위한 기술 개발은 물론 복숭아의 소비 촉진과 해외 수출 확대에도 크게 기여할 것이다.

키워드
경도
보구력
에틸렌
호흡
real-time PCR
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Information
  • Publisher :KOREAN SOCIETY FOR HORTICULTURAL SCIENCE
  • Publisher(Ko) :원예과학기술지
  • Journal Title :Horticultural Science and Technology
  • Journal Title(Ko) :원예과학기술지
  • Volume : 38
  • No :1
  • Pages :32-43
  • Received Date : 2019-09-20
  • Revised Date : 2019-11-06
  • Accepted Date : 2019-11-17
  • DOI :https://doi.org/10.7235/HORT.20200004
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